User:Allancrossman

From 2011.igem.org

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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
 
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==Personal TODO list==
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<p class="h1">Allan Crossman</p>
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* What happened to our primers e.g. for xylA?
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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
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* What is density of displayed INP?
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* Plan for zipper system.
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* Plan for pIII beads reactor?
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* Can we suppress the hover-over thing for pages with no hard words?
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* The hover-over thing fails on IE7?
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* Add hints.
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==Notes to self==
==Notes to self==
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* We MUST have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
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* [[Judging]]
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* ''inaZ'' is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
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* [https://igem.org/Results?year=2011 2011 full results]
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* To add hints, edit [[Team:Edinburgh/tech/Hints]] and also add &lt;span class="hardword" id="foo"&gt;foo&lt;/span&gt; in the relevant page.
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* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
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* Assembly page is here: [[Team:Edinburgh/AssemblyContent]]
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* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
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* The bglX part has a PstI site in it. Once fixed, the official part docs will need to be redone.
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* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
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* [[Special:Watchlist]]
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==Video game concept==
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You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?
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==Recombination avoidance test?==
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If INP works, and if we have the money, we could test whether our recombination avoidance strategy actually works, by getting a new version of BBa_K265008 synthesised with synonymous codons, and setting up a construct such that some reporter (e.g. LacZ, YFP, or whatever) would only be expressed if recombination occurred:
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: Promoter - RBS - INP1 - Terminator - Space - INP2 - Reporter
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See if there is any difference between this and:
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: Promoter - RBS - INP1 - Terminator - Space - INP1 - Reporter
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==Stuff others are doing==
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...when cloned into a RecA positive ''E. coli'' strain.
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* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
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* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
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* [https://2011.igem.org/Special:Contributions/mclee Contributions: Lee]
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* [https://2011.igem.org/Special:Contributions/sylvia.ispasani Contributions: Sylvia]
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* [https://2011.igem.org/Special:Contributions/Fionntom Contributions: Fionn]
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* [https://2011.igem.org/Special:Contributions/Di.L Contributions: Di]
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==Lignin degradation==
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==Competitors in our track==
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* Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
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: '''Progress to MIT:'''
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** Lignin peroxidase
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:: [[Team:Edinburgh]] (gold)
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** Manganaese peroxidase
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:: [[Team:Johns_Hopkins]] (bronze)
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** Laccase?
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:: [[Team:UTP-Panama]] (bronze)
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:: [[Team:Washington]] (gold, Americas overall winner)
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:: [[Team:Yale]] (gold, Americas finalist)
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: '''Did not progress:'''
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:: [[Team:Alberta]]
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:: [[Team:Debrecen_Hungary]]
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:: [[Team:HIT-Harbin]]
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:: [[Team:Korea_U_Seoul]]
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:: [[Team:Nevada]]
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:: [[Team:Tec-Monterrey]]
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:: [[Team:Tianjin]]
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:: [[Team:UNAM-Genomics_Mexico]]
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:: [[Team:UST-Beijing]]
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:: [[Team:WashU]]
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Latest revision as of 18:38, 28 October 2011