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Team:EPF-Lausanne/Protocols/Primers preparation
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{{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Initial primer dilution}} | {{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Initial primer dilution}} | ||
| - | + | When receiving new primers, we need to add water in them on order to achieve '''50 microM''' concentration. One rapid method is to: | |
| - | When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to: | + | |
* take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | * take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | ||
* '''spin down the primers tube''' to be sure that all DNA is at the bottom | * '''spin down the primers tube''' to be sure that all DNA is at the bottom | ||
Latest revision as of 19:46, 18 September 2011
Initial primer dilution
Back to protocols.When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to:
- take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water
- spin down the primers tube to be sure that all DNA is at the bottom
- add the calculated amount of water in each tube, then mix them
