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Team:Arizona State
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| - | {{:Team: | + | {{:Team:Arizona State/Templates/main|title=|content= |
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| + | We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition. | ||
| + | <center>[[Image:ASU_tips.png|400px]]</center> | ||
== Abstract == | == Abstract == | ||
| - | Our | + | <p>Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.</p> |
| - | ''[[Team:Arizona State/Project|more]]'' | + | ''[[Team:Arizona State/Project/Introduction|more]]'' |
== What is CRISPR? == | == What is CRISPR? == | ||
| - | '''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell. | + | <p>'''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.</p> |
| - | + | <center> | |
| - | ''[[Team:Arizona State/Project/CRISPR| | + | [[Image:ASU Crispr basic.png|600px|Basic mechanism]]<br> |
| - | + | A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. '''[[Team:Arizona State/Project/CRISPR|More information]]'''. | |
| - | + | </center> | |
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Latest revision as of 03:17, 29 September 2011
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 We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.
 AbstractClustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future. What is CRISPR?Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.
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