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Team:Paris Liliane Bettencourt/Notebook/2011/07/13/
From 2011.igem.org
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| - | '''Glycerol from lacO/tetO plasmids :''' | + | ==Kevin== |
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| + | '''Glycerol from lacO/tetO plasmids :''' <br> | ||
*Centrifugation 10min - 4000 RPM | *Centrifugation 10min - 4000 RPM | ||
*Put liquid on trash and vortex it | *Put liquid on trash and vortex it | ||
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*Put it on cryovial tube (1,5mL on each tube -20°C / -80°C) | *Put it on cryovial tube (1,5mL on each tube -20°C / -80°C) | ||
| - | '''Cultur of glycerols :''' | + | '''Cultur of glycerols :'''<br> |
10mL LB + 10μL Antibiotics<br> | 10mL LB + 10μL Antibiotics<br> | ||
(List on 2011/07/12) | (List on 2011/07/12) | ||
Latest revision as of 16:00, 27 August 2011
Kevin
Glycerol from lacO/tetO plasmids :
- Centrifugation 10min - 4000 RPM
- Put liquid on trash and vortex it
- Resuspend with LB + Glycerol 20% (3mL on each tube)
- Put it on cryovial tube (1,5mL on each tube -20°C / -80°C)
Cultur of glycerols :
10mL LB + 10μL Antibiotics
(List on 2011/07/12)
