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Team:Freiburg/Notebook/20 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
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| - | ==Lysis cassette test== | + | <div id="notebook-page-header"> |
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/19_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 20 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/21_June">Next entry</a> | ||
| + | </div> | ||
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| + | ==<span style="color:orange;">Lysis cassette</span>== | ||
| + | ===Lysis cassette test=== | ||
'''Investigators: Theo''' | '''Investigators: Theo''' | ||
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5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed. | 5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed. | ||
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==Digestion of PR1-PR9== | ==Digestion of PR1-PR9== | ||
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'''Investigators: Julia, Theo''' | '''Investigators: Julia, Theo''' | ||
| - | Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9. | + | Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9.<br/> |
| - | Enzyme 1: XhoI | + | Enzyme 1: XhoI<br/> |
| - | Enzyme 2: PstI | + | Enzyme 2: PstI<br/> |
Samples were then loaded onto a gel. | Samples were then loaded onto a gel. | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:21, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
