Team:Freiburg/Notebook/20 June

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=='''15. Labday'''==
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==Lysis cassette test==
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/19_June">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 20 June </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/21_June">Next entry</a>
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==<span style="color:orange;">Lysis cassette</span>==
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===Lysis cassette test===
'''Investigators: Theo'''
'''Investigators: Theo'''
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<span style="color:blue;"> Comments: cells were used with the lysis cassette transformed on Saturday</span>
<span style="color:blue;"> Comments: cells were used with the lysis cassette transformed on Saturday</span>
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Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C.
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5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed.
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==Digestion of PR1-PR9==
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'''Investigators: Julia, Theo'''
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Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9.<br/>
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Enzyme 1: XhoI<br/>
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Enzyme 2: PstI<br/>
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Samples were then loaded onto a gel.
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{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:21, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Lysis cassette

Lysis cassette test

Investigators: Theo

Comments: cells were used with the lysis cassette transformed on Saturday

5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed.

Digestion of PR1-PR9

Investigators: Julia, Theo

Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9.
Enzyme 1: XhoI
Enzyme 2: PstI
Samples were then loaded onto a gel.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/20_June"