Team:Freiburg/Notebook/8 June

From 2011.igem.org

(Difference between revisions)
(→Protein modelling and amplification of parts)
 
(20 intermediate revisions not shown)
Line 1: Line 1:
-
==Protein modelling and amplification of parts==
+
{{:Team:Freiburg/Templates/header}}
-
'''Investigators:<br/>''' Protein modelling with pymol: Rüdiger<br/> Amplification of different parts: Julia and Jacob
+
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/7_June">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 8 June </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/9_June">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
 
 +
==<span style="color:grey;">Precipitator</span>==
 +
===Protein modelling with pymol===
 +
 
 +
'''Investigators: Rüdiger'''
 +
<br/>
 +
<br/>
 +
 
 +
==Amplification of different parts==
 +
 
 +
'''Investigators: Julia and Jacob'''
antibiotics plates:<br/>
antibiotics plates:<br/>
-
1oo mg/ml Ampizilin (100 microliter per plate)<br/>
+
1oo mg/ml ampicillin (amp) (100 microliter per plate)<br/>
-
1oo mikroliter H2O + 2 microliter Cm<br/>
+
1oo mikroliter H2O + 2 microliter canamycin (cm)<br/>
-
1oo mikroliter H2O + 40 microliter Tet<br/>
+
1oo mikroliter H2O + 40 microliter tetracyclin(tet)<br/>
-
1oo mikroliter H2O + 10 microliter Kan<br/>
+
1oo mikroliter H2O + 10 microliter kanamycin (kn)<br/>
 +
 
-
{| border="1" align="center" style="text-align:center;"
+
{| border="1" style="text-align:left;"
|'''part'''
|'''part'''
|'''location(p=plate)  '''
|'''location(p=plate)  '''
Line 27: Line 51:
|pSB1K3
|pSB1K3
|P1, 5A
|P1, 5A
-
|kana
+
|kan
-
|
+
|high copy BioBrick assemby plasmid
|-
|-
|pSB1A3
|pSB1A3
|P1, 1G
|P1, 1G
|amp
|amp
-
|
+
|high copy BioBrick assemby plasmid
|-
|-
|pSB1C3
|pSB1C3
|P1,3A
|P1,3A
-
|chloramphenicol
+
|cm
-
|
+
|high copy BioBrick assemby plasmid
|-
|-
|pSB1T3
|pSB1T3
|P1, 7A
|P1, 7A
|tet
|tet
-
|
+
|high copy BioBrick assemby plasmid
|-
|-
|J23104
|J23104
|P1, 18K
|P1, 18K
|
|
 +
|promotor
 +
|-
 +
|J23110
 +
|P1, 20 C
|
|
 +
|promotor
 +
|-
 +
|J23116
 +
|P1, 20M
 +
|
 +
|promotor
 +
|-
 +
|B0034
 +
|P1, 2M
 +
|
 +
|RBS (strong)
 +
|-
 +
|B0032
 +
|P1, 2 I
 +
|
 +
|RBS.3 (medium)
 +
|-
 +
|B0031
 +
|P1, 2G
 +
|
 +
|RBS.2 (weak)
|}
|}
 +
 +
Transformation of parts in competent cells and plating them out on plates with specific antiobiotic resistance.
 +
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:18, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Precipitator

Protein modelling with pymol

Investigators: Rüdiger

Amplification of different parts

Investigators: Julia and Jacob

antibiotics plates:
1oo mg/ml ampicillin (amp) (100 microliter per plate)
1oo mikroliter H2O + 2 microliter canamycin (cm)
1oo mikroliter H2O + 40 microliter tetracyclin(tet)
1oo mikroliter H2O + 10 microliter kanamycin (kn)


part location(p=plate) resistance info
BBa_K098995 P3, 1E amp heat sensitive cI QPI with high promoter
BBa_K112022 P3, 24E amp Lambda phage lysis device - no promoter, This part is in BBb Format.
It is flanked by BamHI and BglII sites instead of XbaI and SpeI.
pSB1K3 P1, 5A kan high copy BioBrick assemby plasmid
pSB1A3 P1, 1G amp high copy BioBrick assemby plasmid
pSB1C3 P1,3A cm high copy BioBrick assemby plasmid
pSB1T3 P1, 7A tet high copy BioBrick assemby plasmid
J23104 P1, 18K promotor
J23110 P1, 20 C promotor
J23116 P1, 20M promotor
B0034 P1, 2M RBS (strong)
B0032 P1, 2 I RBS.3 (medium)
B0031 P1, 2G RBS.2 (weak)

Transformation of parts in competent cells and plating them out on plates with specific antiobiotic resistance.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/8_June"