Team:Wageningen UR/Notebook/Proj2/July

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== July - Fungal Track 'n Trace ==  
== July - Fungal Track 'n Trace ==  
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[[File:Jul2.png]]
[[File:Jul2.png]]
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'''4 July'''
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'''July 4'''
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4 Erlenmeyers containing transformation medium were inoculated with either 0.5*106, 1*106, 5*106 or 107 spores/mL and were grown overnight at 30˚C. This time fresh spores were used which were derived from a spore plate that had grown for 4 days.
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Inoculated 4 Erlenmeyers containing transformation medium with either 0.5*106, 1*106, 5*106 or 107 spores/mL and grew them overnight at 30˚C. This time fresh spores were used which were derived from a spore plate that had grown for 4 days.
 
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'''July 5'''
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'''5 July'''
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Dr. LdG showed us how to recover protoplasts from mycelium. He used the mycelium derived from the 1*106 spores/mL medium, as this one showed the least amount of pellets and decent growth. Buffers with ranging osmotic values were prepared to see whether this had any effect on protoplast formation. This time the protoplast recovery resulted in approximately 80 aliquots.
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Dr. LdG showed me how to recover protoplasts from mycelium. He used the mycelium derived from the 1*106 spores/mL medium, as this one showed the least amount of pellets and decent growth. Myself, Youri and David were assisting him. Buffers with ranging osmotic values were prepared to see whether this had any effect on protoplast formation. This time the protoplast recovery resulted in approximately 80 aliquots.
 
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'''July 6'''
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'''6 July'''
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Again, a test was carried out to check the amount of viable protoplasts in the protoplast solutions and to check for mycelia contamination. Results will be visible on Friday, July 8.
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Again, a test was carried out to check the amount of viable protoplasts in the protoplast solutions and to check for mycelia contamination. Results will be visible on Friday, 8th July.
 
[https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/June Previous Month]
[https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/June Previous Month]

Latest revision as of 21:03, 18 August 2011

Building a Synchronized Oscillatory System





July - Fungal Track 'n Trace



Jul2.png

July 4

4 Erlenmeyers containing transformation medium were inoculated with either 0.5*106, 1*106, 5*106 or 107 spores/mL and were grown overnight at 30˚C. This time fresh spores were used which were derived from a spore plate that had grown for 4 days.


July 5

Dr. LdG showed us how to recover protoplasts from mycelium. He used the mycelium derived from the 1*106 spores/mL medium, as this one showed the least amount of pellets and decent growth. Buffers with ranging osmotic values were prepared to see whether this had any effect on protoplast formation. This time the protoplast recovery resulted in approximately 80 aliquots.


July 6

Again, a test was carried out to check the amount of viable protoplasts in the protoplast solutions and to check for mycelia contamination. Results will be visible on Friday, July 8.


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