"
Team:EPF-Lausanne/Protocols/Transformation
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:EPF-Lausanne/Templates/Header|title=Transformation}} == Materials == == Protocol == {{:Team:EPF-Lausanne/Templates/Footer}}") |
(→Additional notes) |
||
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:EPF-Lausanne/Templates/ | + | {{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Transformation}} |
| + | Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf 2010 protocol] | ||
| - | == | + | == Additional notes == |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | * The protocol specifies to ask Henrike for plasmid quantities. Here is her answer: | ||
| + | ** For relatively pure plasmids, 1 ul is enough. | ||
| + | ** For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul. | ||
| + | ** If you are conducting the heat shock in an eppendorf tube, use 45 seconds instead of 30. | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Latest revision as of 12:47, 11 August 2011
Transformation
Back to protocols.Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: 2010 protocol
Additional notes
- The protocol specifies to ask Henrike for plasmid quantities. Here is her answer:
- For relatively pure plasmids, 1 ul is enough.
- For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul.
- If you are conducting the heat shock in an eppendorf tube, use 45 seconds instead of 30.
