Team:Grenoble/Projet/Biobricks

From 2011.igem.org

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Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC
Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC
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<div class="body">
<div class="body">
<div class="left">
<div class="left">
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<div class="biobricks">
 
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<h1>Our Biobricks:</h1>
 
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<div class="blocbackground">
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<div class="blocbackground">
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<h1>Biobricks</h1>
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<ul>
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<p>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composites parts we send to the registry.</li>
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<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composite parts we send to the registry.
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</p>
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</div class="blocbackground">
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</ul>
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</html>
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<center>
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<groupparts>iGEM011 Grenoble</groupparts>
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</center>
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              <html>
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<div class="blocbackground">
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<ul>
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<li>You can also find in the registry some of our constructions intermediates:</li>
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</ul>
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</p>
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<table>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04602">BBa_S04602</a></td>
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<td><p><strong>RBS-CinR</strong>, BBa_B0034+BBa_C0077 </p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04603">BBa_S04603</a></td>
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<td><p><strong>RBS-TetR</strong>, BBa_B0034+BBa_C0040 </p></td>
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</tr> 
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04606">BBa_S04606</a></td>
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<td><p><strong>RBS-TetR-RBS-CinR</strong>, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04605">BBa_S04605</a></td>
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<td><p><strong>RBS-TetR-RBS-GFP</strong>, BBa_B0034+BBa_C0077+BBa_E0240</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04607">BBa_S04607</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_R0011+BBa_C0077</p></td>
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</tr>
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<tr>
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<td><a href="http://partsregistry.org/Part:BBa_S04618">BBa_S04618</a></td>
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<td><p><strong>pLac-CinR</strong>, BBa_K545006+BBa_I13401</p></td>
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</tr>
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</table>
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</div class="blocbackground">
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</div>
</div>
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                <div class="blocbackground">
 
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                  <table align="center">
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</html>
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<tr>
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<center>
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  <td>
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<groupparts>iGEM011 Grenoble</groupparts>
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      <p align="left">
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</center>
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      MerR-del : K545103.
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<html>
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      MerR (BBa_K346001) without RBS
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      PCR gel
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      Well 1: Positive control (pSB4K5)
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      Well 2: Negative control (Without DNA)
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      Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
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      Confirmed by sequencing
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      </p>
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  </td>
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  <td>
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    <img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/>
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  </td>
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</tr>
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<tr>
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<div class="blocbackground">
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  <td>
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<p>
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    <p align="left">
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You can also find in the registry some of our intermediate constructions:
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    pMerT-GFP: K545002
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</p>
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    Original parts: K346002, E0240
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-
    PCR gel
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<table>
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    Well 1 and 2: Failed cloning
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<tr>
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    Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
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<td><a href="http://partsregistry.org/Part:BBa_S04602">BBa_S04602</a></td>
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    Confirmed by sequencing
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<td><p><strong>RBS-CinR</strong>, BBa_B0034+BBa_C0077 </p></td>
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    </p>
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</tr>
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  </td>
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<tr>
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  <td>
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<td><a href="http://partsregistry.org/Part:BBa_S04603">BBa_S04603</a></td>
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<img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/>
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<td><p><strong>RBS-TetR</strong>, BBa_B0034+BBa_C0040 </p></td>
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  </td>
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</tr> 
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</tr>
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<tr>
 +
<td><a href="http://partsregistry.org/Part:BBa_S04606">BBa_S04606</a></td>
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<td><p><strong>RBS-TetR-RBS-CinR</strong>, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077</p></td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://partsregistry.org/Part:BBa_S04605">BBa_S04605</a></td>
 +
<td><p><strong>RBS-TetR-RBS-GFP</strong>, BBa_B0034+BBa_C0077+BBa_E0240</p></td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://partsregistry.org/Part:BBa_S04607">BBa_S04607</a></td>
 +
<td><p><strong>pLac-CinR</strong>, BBa_R0011+BBa_C0077</p></td>
 +
</tr>
 +
<tr>
 +
<td><a href="http://partsregistry.org/Part:BBa_S04618">BBa_S04618</a></td>
 +
<td><p><strong>pLac-CinR</strong>, BBa_K545006+BBa_I13401</p></td>
 +
</tr>  
 +
</table>
 +
</div>
 +
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<tr>
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<div class="blocbackground">
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  <td>
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<table align="center">
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    <p align="left">
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<tr>
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    pCin-CrtEBI (K545000)
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<td>
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    Originally: R0077, K274100
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<p align="left">
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    PCR gel
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<center><h5>MerR-del (K545103)</h5></center><br/><br/>
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    Expected at 3500pb, obtain between 3000 and 4000pb
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    Confirmed by sequencing
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MerR (BBa_K346001) without RBS<br/>
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  </td>
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PCR gel<br/><br/>
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  <td>
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<img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/>
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Well 1: Positive control (pSB4K5)<br/>
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  </td>
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Well 2: Negative control (Without DNA)<br/>
-
</tr>
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Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>pMerT-GFP (K545002)</h5></center><br/><br/>
 +
 +
Original parts: K346002, E0240<br/>
 +
PCR gel<br/><br/>
 +
 +
Well 1: MW<br/>
 +
Well 2: empty well<br/>
 +
Well 3 and 4: clonning failure<br/>
 +
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>
 +
 +
Original parts: R0077, K274100<br/>
 +
PCR gel<br/><br/>
 +
 +
Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)<br/>
 +
Well 4: MW<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
 +
 +
Original parts:  J23119, B0034 C0077<br/>
 +
Restriction gel<br/><br/>
 +
 +
Wells 1 and 2: pConst-CinR(expected at 950pb)<br/>
 +
Wells 4 and 5: cloning failure<br/>
 +
Wells 3 and 6: empty wells<br/>
 +
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
 +
 +
Original parts: R0011, E0240<br/>
 +
Restriction gel<br/><br/>
 +
 +
<center><h5>pConst-GFP (K545001)</h5></center><br/><br/>
 +
 +
Original parts: J23119, E0240<br/>
 +
Restriction gel<br/><br/>
 +
 +
Well 1: pLac-CinR (expected at 950pb)<br/>
 +
Well 2: pConst-CinR (expected at 900pb)<br/>
 +
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
 +
 +
Original parts: B0034, C0077<br/>
 +
PCR gel<br/><br/>
 +
 +
Well 1: RBS-CinR (expected at 1000)<br/>
 +
Wells 2, 3 and 4: clonning failure<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p align="left">
 +
<center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
 +
 +
Original parts: B0034, C0040<br/>
 +
PCR gel<br/><br/>
 +
 +
well 1: RBS-TetR (expected at 950pb)<br/><br/>
 +
 +
Confirmed by sequencing<br/>
 +
</p>
 +
</td>
 +
<td>
 +
<center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center>
 +
</td>
 +
</tr>
 +
</table>
 +
</div>
-
<tr>
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</div>
-
  <td>
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-
    <p align="left">
+
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    pConst-RBS-CinR (K545004)
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-
    Originally J23119, B0034 C0077
+
-
    Restriction gel
+
-
    Well 1, 2: failed cloning
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-
    Well 3, 4: pConst-CinR(expected at 950pb)
+
-
    Confirmed by sequencing
+
-
  </td>
+
-
  <td>
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<img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/>
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-
  </td>
+
-
</tr>
+
-
<tr>
+
</div>
-
  <td>
+
-
    <p align="left">
+
-
    pLac-CinR (S04607)
+
-
    Originally R0011, E0240
+
-
    pConst-GFP (K545001)
+
-
    Originally J23119, E0240
+
-
    Restriction gel
+
-
    Well 1: pLac-CinR (expected at 950pb)
+
-
    Well 2: pConst-CinR (expected at 900pb)
+
-
    Confirmed by sequencing
+
-
  </td>
+
-
  <td>
+
-
<img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/>
+
-
  </td>
+
-
</tr>
+
-
<tr>
 
-
  <td>
 
-
    <p align="left">
 
-
    RBS-CinR (S04602)
 
-
    Originally B0034, C0077
 
-
    PCR gel
 
-
    Well 1: RBS-CinR (expected at 1000)
 
-
    Confirmed by sequencing
 
-
  </td>
 
-
  <td>
 
-
<img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/>
 
-
  </td>
 
-
</tr>
 
-
<tr>
 
-
  <td>
 
-
    <p align="left">
 
-
    RBS-TetR (S04603)
 
-
    Originally B0034, C0040
 
-
    PCR gel
 
-
    RBS-TetR (expected at 950pb)
 
-
    Confirmed by sequencing
 
-
  </td>
 
-
  <td>
 
-
<img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/>
 
-
  </td>
 
-
</tr>
 
-
 
-
</table>
 
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              </div>
 
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</div>
 
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</div>
 
</html>
</html>
{{:Team:Grenoble/Design/pied}}
{{:Team:Grenoble/Design/pied}}

Latest revision as of 00:05, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Biobricks

Here you can find all our news and composite parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

You can also find in the registry some of our intermediate constructions:

BBa_S04602

RBS-CinR, BBa_B0034+BBa_C0077

BBa_S04603

RBS-TetR, BBa_B0034+BBa_C0040

BBa_S04606

RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605

RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607

pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618

pLac-CinR, BBa_K545006+BBa_I13401

MerR-del (K545103)


MerR (BBa_K346001) without RBS
PCR gel

Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)

Confirmed by sequencing

pMerT-GFP (K545002)


Original parts: K346002, E0240
PCR gel

Well 1: MW
Well 2: empty well
Well 3 and 4: clonning failure
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)

Confirmed by sequencing

pCin-CrtEBI (K545000)


Original parts: R0077, K274100
PCR gel

Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)
Well 4: MW

Confirmed by sequencing

pConst-RBS-CinR (K545004)


Original parts: J23119, B0034 C0077
Restriction gel

Wells 1 and 2: pConst-CinR(expected at 950pb)
Wells 4 and 5: cloning failure
Wells 3 and 6: empty wells
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

pLac-CinR (S04607)


Original parts: R0011, E0240
Restriction gel

pConst-GFP (K545001)


Original parts: J23119, E0240
Restriction gel

Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

RBS-CinR (S04602)


Original parts: B0034, C0077
PCR gel

Well 1: RBS-CinR (expected at 1000)
Wells 2, 3 and 4: clonning failure

Confirmed by sequencing

RBS-TetR (S04603)


Original parts: B0034, C0040
PCR gel

well 1: RBS-TetR (expected at 950pb)

Confirmed by sequencing

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