Team:HokkaidoU Japan

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         <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
         <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
             <span class="hokkaidou-description">
             <span class="hokkaidou-description">
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                 The HokkaidoU_Japan is composed of 16 students and 3 instructors
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                 The HokkaidoU_Japan is formed by 16 students and 3 instructors
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         <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
         <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
             <span class="hokkaidou-description">
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                 We tried to make advancement of E. coli-based protein injection system which we developed on iGEM 2010
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                 We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
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         <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
         <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
             <span class="hokkaidou-description">
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                 The record of our plenty of failures and a bit of achievement
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                 The record of our many failures and a bit of achievement
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         <li id="hokkaidou-index-safety"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
         <li id="hokkaidou-index-safety"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
             <span class="hokkaidou-description">
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                 Answers to official safety questions
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                 Addressing safety concerns
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         <li id="hokkaidou-index-humanpractice"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
         <li id="hokkaidou-index-humanpractice"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
             <span class="hokkaidou-description">
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                 We held a science art gallery "BIO ART"
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                 Science art gallery "BIO ART"
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     <h2>Abstract</h2>
     <h2>Abstract</h2>
     <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
     <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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     <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it,　we tried to further characterize this system by injecting a library of protein domains.</p>
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     <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
     <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
     <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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* Hokuto Kohki Engineering Co,. Ltd.
* Hokuto Kohki Engineering Co,. Ltd.
* COSMO BIO Co., Ltd.
* COSMO BIO Co., Ltd.
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* AMINO　UP　CHEMICAL Co., Ltd.
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* AMINO UP CHEMICAL Co., Ltd.
* Mendel Workshop Co., Ltd.
* Mendel Workshop Co., Ltd.
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{{Team:HokkaidoU_Japan/footer}}

Latest revision as of 09:59, 15 December 2011

Contents

  • HokkaidoU_Japan 2011

  • Welcome to the BIOxART Gallery!!

  • Be careful not to contaminate...

  • Meeting, meeting and MEETING!

  • Dr. E. coli get ready for the job

  • BIOxART, please feel free to look around

  • Experiment, experiment and EXPERIMENT!

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

BioArt

We held the new type science art gallery "Bio Art" at the center of Sapporo city.

This gallery is not only for our team.You can also enjoy Bio Art on this Web page. You can watch more detail in Human Practice page.

Acknowledgements

Laboratory and Personnel

  • Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
Ken-ichi Yamazaki and Members of Yamazaki Lab
  • Molecular Biology, Institute for Genetic Medicine, Hokkaido University
Hisatoshi Shida
  • Nikon Imaging Center
Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
  • Laboratory of Molecular and Cellular Biophysics, Hokkaido University
Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
  • Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
Taizo Motomura
  • Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
Isao Tanaka
  • Research Institute for Electronic Science, Hokkaido University

Academic Institutions

  • Salmonella Genetic Stock Center/University of Calgary
  • Graduate School of Environmental Earth Science
  • Department of biological science/School of Sciense
  • Institute for Genetic Medicine

Companies

  • Primary Cell Co., Ltd.
  • ECONiXE Co., Ltd.
  • Hokuto Kohki Engineering Co,. Ltd.
  • COSMO BIO Co., Ltd.
  • AMINO UP CHEMICAL Co., Ltd.
  • Mendel Workshop Co., Ltd.
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