Team:Copenhagen/Project

From 2011.igem.org

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<!--- The Team --->
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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<!-- Left side -->
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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<!--- The Mission, Experiments --->
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  <br>
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    Table of Contents<br><br>
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<ul>
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    <li><a href="https://2011.igem.org/Team:Copenhagen/Project/Experimental" CLASS=leftbar>Experimental Design</a></li>
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    <li><a href="https://2011.igem.org/Team:Copenhagen/Protocol" CLASS=leftbar>Protocols</a></li>
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    <li><a href="https://2011.igem.org/Team:Copenhagen/Notebook" CLASS=leftbar>Notebook</a></li>
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<li><a href="https://2011.igem.org/Team:Copenhagen/Project/Data" CLASS=leftbar>Data Page</a></li>
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<li><a href="https://2011.igem.org/Team:Copenhagen/Parts" CLASS=leftbar>Parts</a></li>
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<li><a href="https://2011.igem.org/Team:Copenhagen/Results" CLASS=leftbar>Results</a></li>
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<li><a href="https://2011.igem.org/Team:Copenhagen/Collaboration" CLASS=leftbar>Collaboration</a></li>
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<li><a href="https://2011.igem.org/Team:Copenhagen/Attributions" CLASS=leftbar>Attribution</a></li>
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!align="center"|[[Team:Copenhagen|Home]]
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<br>
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!align="center"|[[Team:Copenhagen/Team|Team]]
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<br>
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Copenhagen Official Team Profile]
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<br>
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!align="center"|[[Team:Copenhagen/Project|Project]]
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<br>
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!align="center"|[[Team:Copenhagen/Parts|Parts Submitted to the Registry]]
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<br>
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!align="center"|[[Team:Copenhagen/Modeling|Modeling]]
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!align="center"|[[Team:Copenhagen/Notebook|Notebook]]
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!align="center"|[[Team:Copenhagen/Safety|Safety]]
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!align="center"|[[Team:Copenhagen/Attributions|Attributions]]
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<b><center>CyperMan to the <br><br>
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Rescue</center></b></font>
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<p>&nbsp;</p>
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<p>&nbsp;</p>
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<p align="justify">
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<p align = left><strong>CyperMan</strong></p>
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<p>A CYP is an enzyme with hydroxylation powers. The hydroxylation performed by CYPs are expensive to produce with conventional chemical methods and it can therefore be greatly beneficial to utilize our CyperMans as little synthetic biology factories.<a href="https://2011.igem.org/Team:Copenhagen/Project/Cytochrome" style="text-decoration:none; color:blue;"> Read more.</a><br></p>
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<br>
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We have been working on two parallel projects, with two different aims. They are described in more detail below. The project regarding the human p450's was unfortunately put to a halt. This is why our focus has been on the project involving the cytochrome p450's from plants (the "Kill fungi" project).
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<br>
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<br>
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<p align = left><strong>CyperMan - Kills fungus</strong></p>
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<table class="https://static.igem.org/mediawiki/2011/4/4e/Til_siden.png" align="left">
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<tr><td><img src="https://static.igem.org/mediawiki/2011/4/4e/Til_siden.png"  width="175px"></td></tr>
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<p align = "right">We hope to be able to develop bacteria that can kill fungus.<br>We will introduce a biological system to ''E.coli'' utilizing the plant cytochrome p450 79’s ability to produce small molecules called oximes which are toxic to fungus, as they inhibit the ability of the mitochondrial peroxidases to breakdown potentially harmful hydrogen peroxides. The oximes are produced from aminoacids - phenylalanine, tyrosine and tryptophan.
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<a href="https://2011.igem.org/Team:Copenhagen/Project/Fungus" style="text-decoration:none; color:blue;"> Read more.</a><br></p>
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<br>
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<p align = right><strong>CyperMan - Saves the Fish</strong>
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<table class="https://static.igem.org/mediawiki/2011/8/88/CyperMan_fisk.jpg" align="right">
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<tr><td><img src="https://static.igem.org/mediawiki/2011/8/88/CyperMan_fisk.jpg"  width="180px"></td></tr>
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</table>
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== '''Overall project''' ==
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We hope to find and possibly optimize a biological system which can be introduced in bacteria and lead to the removal of PPCP (pharmaceutical  and personal care products) pollutants in water.
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We intend to transform E.coli bacteria with different combinations of the several membrane bound human cytochrome p450 and examine our different cytochrome p450 transformed E.coli bacteria and their ability to impede estrogenic activity water. <a href="https://2011.igem.org/Team:Copenhagen/Project/Water" style="text-decoration:none; color:blue;"> Read more.</a><br></p>
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Your abstract
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<br>
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<br>
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<strong>Impact factors:</strong>
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<br>
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<br>
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Our project has the aim to characterize different subfamilies of Cytochrome p450 as a BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in these particular proteins. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of different cytochrome p450 to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of p450's and thus contribute to the formation of new biological systems – further expanding the field of synthetic biology.
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== Project Details==
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<div class="dom" style="margin-top:-3px; border:1px white;" ><cellpadding=20px>
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<p style="color: darkblue; font-size:1.5em; text-indent:0px; text-left;"><font face="constantia"><u><strong>iGEM Medals Requirements</strong></u></font></p><br>
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<p style="font-size:1.3em; text-indent:0px; text-align:left; color:#614e1a; border:2px;"><strong>Bronze</strong></p>
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=== Part 2 ===
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Team registration<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Complete Judgment form.<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2011.igem.org/Team:Copenhagen" style="text-decoration:none; color:black;"> Team Wiki.</a>
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<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Plan to present a poster and a talk at the iGEM Jamboree<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2011.igem.org/Team:Copenhagen/Parts" style="text-decoration:none; color:black;"> At least one new submitted and well-characterized standard BioBrick Part or Device.</a><br>
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<br></p>
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<p style="font-size:1.3em; text-indent:0px; text-align:left;color: #AAAAAA;"><strong>Silver</strong></p>
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=== The Experiments ===
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<p style="text-indent:0px;">
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> <a href="https://2011.igem.org/Team:Copenhagen/Results" style="text-decoration:none; color:black;">Demonstrate that at least one new BioBrick Part of your own design and construction works as expected.</a><br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> <a href="https://2011.igem.org/Team:Copenhagen/Results" style="text-decoration:none; color:black;">Characterize the operation of at least one new BioBrick Part..</a><br>
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</p>
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<br>
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<p style="font-size:1.3em; text-indent:0px; text-align:left;color: #ffd600;"><strong>Gold</strong></p>
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<p> For Gold Medal, either of the following must be checked </p>
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=== Part 3 ===
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<p style="text-indent:0px;">
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<IMG SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMhTqQbWTSI/AAAAAAAAAoQ/tU6xh9B91iw/caseCocherEmpty.PNG"/> Improve an existing BioBrick Part or Device. <br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2011.igem.org/Team:Copenhagen/Collaboration" style="text-decoration:none; color:black;"> Help another iGEM team </a><br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2011.igem.org/Team:Copenhagen/Ethics" style="text-decoration:none; color:black;"> Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation </a> .<br>
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<br>
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</p>
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</div>
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</td>
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== Results ==
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<font face="constantia" size="1" font color="#FFFFFF">
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Comments or questions to the team? Please mail us at igemcopenhagen@gmail.com
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Latest revision as of 14:26, 18 September 2011



Table of Contents
CyperMan to the

Rescue

 

 

CyperMan

A CYP is an enzyme with hydroxylation powers. The hydroxylation performed by CYPs are expensive to produce with conventional chemical methods and it can therefore be greatly beneficial to utilize our CyperMans as little synthetic biology factories. Read more.


We have been working on two parallel projects, with two different aims. They are described in more detail below. The project regarding the human p450's was unfortunately put to a halt. This is why our focus has been on the project involving the cytochrome p450's from plants (the "Kill fungi" project).

CyperMan - Kills fungus

We hope to be able to develop bacteria that can kill fungus.
We will introduce a biological system to ''E.coli'' utilizing the plant cytochrome p450 79’s ability to produce small molecules called oximes which are toxic to fungus, as they inhibit the ability of the mitochondrial peroxidases to breakdown potentially harmful hydrogen peroxides. The oximes are produced from aminoacids - phenylalanine, tyrosine and tryptophan. Read more.


CyperMan - Saves the Fish

We hope to find and possibly optimize a biological system which can be introduced in bacteria and lead to the removal of PPCP (pharmaceutical and personal care products) pollutants in water. We intend to transform E.coli bacteria with different combinations of the several membrane bound human cytochrome p450 and examine our different cytochrome p450 transformed E.coli bacteria and their ability to impede estrogenic activity water. Read more.



Impact factors:

Our project has the aim to characterize different subfamilies of Cytochrome p450 as a BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in these particular proteins. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of different cytochrome p450 to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of p450's and thus contribute to the formation of new biological systems – further expanding the field of synthetic biology.


iGEM Medals Requirements


Bronze

Team registration
Complete Judgment form.
Team Wiki.
Plan to present a poster and a talk at the iGEM Jamboree
At least one new submitted and well-characterized standard BioBrick Part or Device.

Silver

Demonstrate that at least one new BioBrick Part of your own design and construction works as expected.
Characterize the operation of at least one new BioBrick Part..


Gold

For Gold Medal, either of the following must be checked

Improve an existing BioBrick Part or Device.
Help another iGEM team
Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation .
Comments or questions to the team? Please mail us at igemcopenhagen@gmail.com

Retrieved from "http://2011.igem.org/Team:Copenhagen/Project"