Team:Peking S/project/wire/matrix
From 2011.igem.org
(→Orthogonal activating matrix) |
|||
(2 intermediate revisions not shown) | |||
Line 20: | Line 20: | ||
Aiming at effective and discrete communications in a multicellular genetic network, orthogonality of the 'chemical wires' is essential. Thus we carried out a series of experiments checking the signaling orthogonality of 'chemical wires' in our toolbox. | Aiming at effective and discrete communications in a multicellular genetic network, orthogonality of the 'chemical wires' is essential. Thus we carried out a series of experiments checking the signaling orthogonality of 'chemical wires' in our toolbox. | ||
- | <center>[[File:signalingOrthogonal.png| | + | <center>[[File:signalingOrthogonal.png|450px]]</center> |
- | ''Figure 1 A graphical representation of the relative activation fold associated with five | + | ''Figure 1 A graphical representation of the relative activation fold associated with five receiver systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is 1:100 dilution of the overnight culture broth. '' |
<center>'''Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)''' | <center>'''Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)''' | ||
Line 39: | Line 39: | ||
[[File:SR_Matrix_table3.png|600px]]</center> | [[File:SR_Matrix_table3.png|600px]]</center> | ||
+ | |||
+ | |||
+ | |||
+ | <html> | ||
+ | <a href="#top">Top↑ | ||
+ | </div> | ||
+ | |||
+ | </html> | ||
+ | <br> | ||
+ | <br> |
Latest revision as of 01:13, 6 October 2011
Template:Https://2011.igem.org/Team:Peking S/bannerhidden Template:Https://2011.igem.org/Team:Peking S/back2
Template:Https://2011.igem.org/Team:Peking S/bannerhidden
Chemical Wire Toolbox
Introduction|Harvesting ‘Chemical Wires’ From Nature|Synthesizing Quorum Sensing Inverters|Orthogonal Activating Matrix
Orthogonal activating matrix
Aiming at effective and discrete communications in a multicellular genetic network, orthogonality of the 'chemical wires' is essential. Thus we carried out a series of experiments checking the signaling orthogonality of 'chemical wires' in our toolbox.
![SignalingOrthogonal.png](/wiki/images/7/74/SignalingOrthogonal.png)
Figure 1 A graphical representation of the relative activation fold associated with five receiver systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is 1:100 dilution of the overnight culture broth.
Table 2 Data of the activating matrix, average fold compared with blank samples calculated
Table 3 Data of the activating matrix, activating fold normalized
![SR Matrix table3.png](/wiki/images/b/bf/SR_Matrix_table3.png)