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Team:Tokyo Metropolitan/Notebook/S20
From 2011.igem.org
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Above samples | Above samples | ||
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| + | @restriction emzyme(XbaⅠ, PstⅠ), electrophoresis, gel extraction, electrophoresis for comfirmation, ligation oriTr4,oriTr-GFP 0A,oriTr-GFP | ||
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| + | @ligation pSB3C3,oriTr-GFP | ||
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| + | = -Targeting- = | ||
| + | Colony PCR | ||
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| + | Colony PCR J23100-P0140. | ||
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| + | Electrophoresis | ||
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| + | Electrophoresis colony PCR,but failure. | ||
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| + | Colony PCR | ||
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| + | Colony PCR J23100-K317037-K283030. | ||
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| + | Electrophoresis | ||
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| + | Electrophoresis colony PCR J23100-K317037-K283030 and find binds almost all. | ||
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| + | Colony PCR | ||
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| + | Colony PCR J23100-P0140. | ||
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| + | Preculture | ||
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| + | P0140,J23100 and J23100-K317037-K283030. | ||
Latest revision as of 14:07, 5 October 2011
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-Visualization-
-Ligation
Promoter - suppressed expression system
-Transformation
Above samples
@restriction emzyme(XbaⅠ, PstⅠ), electrophoresis, gel extraction, electrophoresis for comfirmation, ligation oriTr4,oriTr-GFP 0A,oriTr-GFP
@ligation pSB3C3,oriTr-GFP
-Targeting-
Colony PCR
Colony PCR J23100-P0140.
Electrophoresis
Electrophoresis colony PCR,but failure.
Colony PCR
Colony PCR J23100-K317037-K283030.
Electrophoresis
Electrophoresis colony PCR J23100-K317037-K283030 and find binds almost all.
Colony PCR
Colony PCR J23100-P0140.
Preculture
P0140,J23100 and J23100-K317037-K283030.
