Team:Tokyo Metropolitan/Notebook/S22

From 2011.igem.org

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OriTr-GFP-OriTr 139.5 ng/μl
OriTr-GFP-OriTr 139.5 ng/μl
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-Electrophoresis
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Samples which digested one day ago
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-Gel extraction
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Above samples
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-Ligation
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Promoter - suppressed expression system
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-Transformation
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Above samples
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= -Targetting- =
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@Colony PCR J23100-P0140
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@electrophoresis J23100-K317037 yesterday emzimed and find binds
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@gel extraction, but failure
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@electrophoresis J23100-P0140, colony PCR
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find binds
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@preculture J23100-P0140, J23100-K317037

Latest revision as of 13:37, 5 October 2011


-Visualization-

@Colony PCR and electrophoresis pSB1C3+oriTr-GFP

@preculture from master plate of Colony PCR pSB1C3+oriTr-GFP

@plasmid extraction and DNA refine OriTr, OriTr-GFP

@plasmid extraction and DNA refine OriTr-GFP-OriTr

@DNA densitometry

OriTr-GFP 264.9 ng/μl

OriTr 126.9 ng/μl

OriTr-GFP-OriTr 139.5 ng/μl


-Electrophoresis

Samples which digested one day ago


-Gel extraction

Above samples


-Ligation

Promoter - suppressed expression system


-Transformation

Above samples

-Targetting-

@Colony PCR J23100-P0140

@electrophoresis J23100-K317037 yesterday emzimed and find binds

@gel extraction, but failure

@electrophoresis J23100-P0140, colony PCR find binds

@preculture J23100-P0140, J23100-K317037

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S22"