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Team:Tokyo Metropolitan/Notebook/S08
From 2011.igem.org
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Purification of HNS(wt)and electrophoresis and purification of pSB1C3. | Purification of HNS(wt)and electrophoresis and purification of pSB1C3. | ||
Although both concentrations were not enough, try to ligate and transformation HNS(wt) and pSB1C3. | Although both concentrations were not enough, try to ligate and transformation HNS(wt) and pSB1C3. | ||
| + | |||
| + | |||
| + | = -Visualization- = | ||
| + | -Colony PCR | ||
| + | |||
| + | Samples which transformed one day ago. | ||
| + | |||
| + | |||
| + | -Electrophoresis | ||
| + | |||
| + | Above samples | ||
| + | |||
| + | |||
| + | -Preculture | ||
| + | |||
| + | Promoter | ||
| + | |||
| + | Suppressed expression system | ||
| + | |||
| + | RFP | ||
| + | |||
| + | ---------- | ||
| + | |||
| + | @plasmid extraction, DNA refine, DNA densitometry, restriction emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP | ||
| + | |||
| + | @preculture from transformed 5th Aug. | ||
Latest revision as of 13:57, 5 October 2011
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-Speeding Up-
Purification of HNS(wt)and electrophoresis and purification of pSB1C3. Although both concentrations were not enough, try to ligate and transformation HNS(wt) and pSB1C3.
-Visualization-
-Colony PCR
Samples which transformed one day ago.
-Electrophoresis
Above samples
-Preculture
Promoter
Suppressed expression system
RFP
@plasmid extraction, DNA refine, DNA densitometry, restriction emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP
@preculture from transformed 5th Aug.
