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Team:Tokyo Metropolitan/Notebook/S07
From 2011.igem.org
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| - | + | Extracted plasmid from E.coli transformated BBa_J04450, | |
| - | + | ||
| + | Refined by phenol chloroform mixture and ethanol precipitation, | ||
| + | |||
| + | Added restriction enzyme, | ||
| + | |||
| + | HNS(wt)→X,S | ||
| + | |||
| + | BBa_J04450→X,S | ||
| + | |||
| + | |||
| + | = -Targetting- = | ||
| + | |||
| + | @plasmid extraction and DNA refine J23100-P0440 | ||
| + | |||
| + | @colony PCR J23100-K317037 and J23100-P0440 | ||
| + | |||
| + | @electrophoresis<Br> | ||
| + | J23100-K317037: find binds in 29 and 31<Br> | ||
| + | J23100-P0440: find binds almost all<Br> | ||
| + | |||
| + | @preculture<Br> | ||
| + | J23100-K317037 and J23100-P0440 which find binds. | ||
| + | |||
| + | |||
| + | = -Visualization- = | ||
| + | -Ligation | ||
| + | |||
| + | Promoter - suppressed expression system | ||
Latest revision as of 13:22, 5 October 2011
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-Speeding Up-
Extracted plasmid from E.coli transformated BBa_J04450,
Refined by phenol chloroform mixture and ethanol precipitation,
Added restriction enzyme,
HNS(wt)→X,S
BBa_J04450→X,S
-Targetting-
@plasmid extraction and DNA refine J23100-P0440
@colony PCR J23100-K317037 and J23100-P0440
@electrophoresis
J23100-K317037: find binds in 29 and 31
J23100-P0440: find binds almost all
@preculture
J23100-K317037 and J23100-P0440 which find binds.
-Visualization-
-Ligation
Promoter - suppressed expression system
