Team:Tokyo Metropolitan/Notebook/S06

From 2011.igem.org

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Preculture(pSB1C3)
Preculture(pSB1C3)
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= -Targeting- =
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-Colony PCR
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Did colony PCR for J23100-P0440.
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Did not show bands.
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-Ligation
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Ligated for J23100-K317037 and J23100-P0440.
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-Transformation
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Transformed for J23100-K317037, J23100-P0440 and J23100 (control).
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-Preculture
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Spread no. 26 and no.31 for liquid medium with ampicillin from masterplate of J23100-P0440 on 5th September. After, had been incubated at 37 deg C.
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= -Visualization- =
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-Digestion
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Promoter: Spe1
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Suppressed expression system: Xba1 and Spe1
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-Electrophoresis
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Above samples
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-Gel extraction
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Above samples
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-Electrophoresis to check
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Above samples

Latest revision as of 13:21, 5 October 2011

-Speeding Up-

Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect.

Electrophoresis of digested HNS(wt)(XbaI+SpeI),I20260(BlnⅠ).Concentration of both HNS(wt)(XbaI+SpeI) and I20260(BlnⅠ) was low.

Preculture(pSB1C3)


-Targeting-

-Colony PCR

Did colony PCR for J23100-P0440.

Did not show bands.


-Ligation

Ligated for J23100-K317037 and J23100-P0440.


-Transformation

Transformed for J23100-K317037, J23100-P0440 and J23100 (control).


-Preculture

Spread no. 26 and no.31 for liquid medium with ampicillin from masterplate of J23100-P0440 on 5th September. After, had been incubated at 37 deg C.


-Visualization-

-Digestion

Promoter: Spe1

Suppressed expression system: Xba1 and Spe1


-Electrophoresis

Above samples


-Gel extraction

Above samples


-Electrophoresis to check

Above samples

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S06"