Team:Tokyo Metropolitan/Notebook/A30

From 2011.igem.org

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= -Speeding Up- =
 
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Amplified HNS(wt),I20260 with PCR and purified these PCR products.
 
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{{:Team:Tokyo_Metropolitan/Header}}
 
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<html>
 
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<div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;">
 
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</html>
 
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{{:Team:Tokyo_Metropolitan/Header}}
 
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<html>
 
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<div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;">
 
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</html>
 
{{:Team:Tokyo_Metropolitan/Header}}
{{:Team:Tokyo_Metropolitan/Header}}
<html>
<html>
<div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;">
<div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;">
</html>
</html>
 +
= -Speeding Up- =
 +
Amplified HNS(wt),I20260 with PCR and purified these PCR products.
= -Visualization- =
= -Visualization- =
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
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------------------------------------
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@plasmid extraction OriTr
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 +
@DNA refine OriTr
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@DNA densitometry
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@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr，OriTr+GFP
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= -Targeting- =
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-DNA refining
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P0440
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no.1 + no.3 143
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no.4 + no.5 130
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J23100
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no.1 + no.3 170
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no.4 + no.5 208
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K317037
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no.3 + no.5 210
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-Digestion
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P0440xba+pst
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J23100Spe+pst
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K317037xba+pst
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after digested, incubated for 5 hours at 37 deg C.
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-Electrophoresis
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Results
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Only had cut K317037
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-Gel extraction
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Gel extracted for K317037, then DNA refined
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-Mesurement of concentration
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J23100 -> 90
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K317037 no.2 102, no.4 75
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(ng/ul)

Latest revision as of 15:39, 5 October 2011

-Speeding Up-

Amplified HNS(wt),I20260 with PCR and purified these PCR products.

-Visualization-

Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.

@plasmid extraction OriTr

@DNA refine OriTr

@DNA densitometry

@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr，OriTr+GFP


-Targeting-

-DNA refining

P0440

no.1 + no.3 143

no.4 + no.5 130


J23100

no.1 + no.3 170

no.4 + no.5 208


K317037

no.3 + no.5 210


-Digestion

P0440xba+pst

J23100Spe+pst

K317037xba+pst


after digested, incubated for 5 hours at 37 deg C.


-Electrophoresis

Results

Only had cut K317037


-Gel extraction

Gel extracted for K317037, then DNA refined


-Mesurement of concentration

J23100 -> 90

K317037 no.2 102, no.4 75

(ng/ul)

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/A30"