Team:Tokyo Metropolitan/Notebook/A29

From 2011.igem.org

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(Speeding Up)
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= -Speeding Up- =
= -Speeding Up- =
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Amplified HNS(wt) and BBa_I20260 with PCR. Only HNS(wt) was succeeded.
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PCR HNS(wt), BBa_I20260(except GFP) and electrophored.
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Purification of HNS(wt)
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 +
PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.
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As a result, confirmated increase of HNS(wt) but not BBa_I20260.
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HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.
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= -Visualization- =
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Preculture
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Precultured promoter(J23100) and  expression inhibiter(I714051)
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@electrophoresis
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@preculture
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OriTR(J01003)
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= -Targetting- =
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 +
@DNA refine J23200, K317037 and P0440

Latest revision as of 12:30, 5 October 2011

-Speeding Up-

PCR HNS(wt), BBa_I20260(except GFP) and electrophored.

PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.

As a result, confirmated increase of HNS(wt) but not BBa_I20260.

HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.

-Visualization-

Preculture Precultured promoter(J23100) and expression inhibiter(I714051)


@electrophoresis

@preculture

OriTR(J01003)

-Targetting-

@DNA refine J23200, K317037 and P0440