Latest revision as of 12:30, 5 October 2011

-Speeding Up-

PCR HNS(wt), BBa_I20260(except GFP) and electrophored.

PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.

As a result, confirmated increase of HNS(wt) but not BBa_I20260.

HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.

Preculture Precultured promoter(J23100) and expression inhibiter(I714051)

@electrophoresis

@preculture

OriTR(J01003)

@DNA refine J23200, K317037 and P0440

@@ Line 1: / Line 1: @@
-=Speeding Up=
+{{:Team:Tokyo_Metropolitan/Header}}
-Amplified HNS(wt) and BBa_I20260 with PCR. Only HNS(wt) was succeeded.
+<html>
-Purification of HNS(wt)
+<div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;">
+</html>
+= -Speeding Up- =
+PCR HNS(wt), BBa_I20260(except GFP) and electrophored.
+PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.
+As a result, confirmated increase of HNS(wt) but not BBa_I20260.
+HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.
+= -Visualization- =
+Preculture
+Precultured promoter(J23100) and  expression inhibiter(I714051)
+@electrophoresis
+@preculture
+OriTR(J01003)
+= -Targetting- =
+@DNA refine J23200, K317037 and P0440