Team:HokkaidoU Japan

From 2011.igem.org

(Difference between revisions)
 
(65 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:HokkaidoU_Japan/Index/Slideshow}}
{{Team:HokkaidoU_Japan/header}}
{{Team:HokkaidoU_Japan/header}}
-
 
-
{{Team:HokkaidoU_Japan/top}}
 
-
 
<html>
<html>
-
    <script type="text/javascript">
+
<script type="text/javascript">
-
    $(document).ready( function(){  
+
$(document).ready(function(){
-
      $('#lofslidecontent45').lofJSidernews({  
+
    var buttons = {
-
         interval : 4000,
+
        previous: $('#lofslidercontent45 .lof-previous'),
-
         direction       : 'opacitys',  
+
        next: $('#lofslidercontent45 .lof-next')
-
         easing         : 'easeInOutExpo',
+
    };
-
         duration       : 1200,
+
    $obj = $('#lofslidecontent45').lofJSidernews({
-
         auto           : false,
+
         interval: 5000,
-
         maxItemDisplay : 4,
+
         direction: 'opacitys',
-
         navPosition     : 'horizontal',
+
         easing: 'easeInOutExpo',
-
         navigatorHeight : 32,
+
         duration: 1200,
-
         navigatorWidth : 80,
+
         auto: true,
-
         mainWidth       : 980,
+
         maxItemDisplay: 7,
-
         buttons         : {
+
        startItem: 0,
-
            previous    : $('#lofslidecontent45 .lof-previous') ,
+
         navPosition: 'horizontal', // horizontal
-
            next        : $('#lofslidecontent45 .lof-next')
+
         navigatorHeight: 32,
-
        }
+
         navigatorWidth: 80,
-
      });
+
         mainWidth: 780,
-
    });
+
         buttons: buttons
-
    </script>
+
    });
-
    <style>
+
});
-
      @charset "utf-8";
+
</script>
-
      .lof-slidecontent, .lof-slidecontent a {
+
<div id="hokkaidou-left-content">
-
        color:#FFF; 
+
    <ul id="hokkaidou-index-menu">
-
      }
+
        <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
-
      .lof-slidecontent a.readmore{
+
            <span class="hokkaidou-description">
-
        color:#58B1EA;
+
                The HokkaidoU_Japan is formed by 16 students and 3 instructors
-
        font-size:95%; 
+
            </span>
-
      }
+
         </a>
-
      .lof-slidecontent{
+
         </li>
-
         position:relative;
+
         <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
-
         overflow:hidden;
+
            <span class="hokkaidou-description">
-
         border:#F4F4F4 solid 1px;
+
                We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
-
      }
+
            </span>
-
      .lof-slidecontent .preload{
+
         </a>
-
        height:100%;
+
         </li>
-
         width:100%;
+
         <li id="hokkaidou-index-parts"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Parts">Parts
-
         position:absolute;
+
            <span class="hokkaidou-description">
-
         top:0;
+
                Ready-to-inject backbone and Bsa I cloning system
-
        left:0;
+
            </span>
-
        z-index:100000;
+
         </a>
-
        text-align:center;
+
         </li>
-
        background:#FFF
+
         <li id="hokkaidou-index-protocol"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Protocols">Protocol
-
      }
+
            <span class="hokkaidou-description">
-
      .lof-slidecontent .preload div{
+
                General protocols, Primers and methods of infection assay
-
         height:100%;
+
            </span>
-
         width:100%;
+
         </a>
-
         background:transparent url(../images/load-indicator.gif) no-repeat scroll 50% 50%;
+
         </li>
-
      }
+
         <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
-
      .lof-main-outer{
+
            <span class="hokkaidou-description">
-
         position:relative;
+
                The record of our many failures and a bit of achievement
-
         height:100%;
+
            </span>
-
         width:900px;
+
         </a>
-
        z-index:3px;
+
         </li>
-
        overflow:hidden;
+
         <li id="hokkaidou-index-safety"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
-
      }
+
            <span class="hokkaidou-description">
-
      /*******************************************************/
+
                Addressing safety concerns
-
      .lof-main-item-desc{
+
            </span>
-
        z-index:100px;
+
         </a>
-
        position:absolute;
+
         </li>
-
        bottom:50px;
+
         <li id="hokkaidou-index-humanpractice"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
-
         left:0px;
+
            <span class="hokkaidou-description">
-
         width:350px;
+
                Science art gallery "BIO ART"
-
         background: -moz-linear-gradient(top, rgba(0,0,0,0.65) 0%, rgba(0,0,0,0) 100%);
+
            </span>
-
        background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(0,0,0,0.65)), color-stop(100%,rgba(0,0,0,0)));
+
         </a>
-
         background: -webkit-linear-gradient(top, rgba(0,0,0,0.65) 0%,rgba(0,0,0,0) 100%);
+
         </li>
-
         background: -o-linear-gradient(top, rgba(0,0,0,0.65) 0%,rgba(0,0,0,0) 100%);
+
    </ul>
-
         background: -ms-linear-gradient(top, rgba(0,0,0,0.65) 0%,rgba(0,0,0,0) 100%);
+
    <div id="hokkaidou-official-team-logo">
-
filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#a6000000', endColorstr='#00000000',GradientType=0 );
+
         <img src="http://igemhokkaidou.files.wordpress.com/2011/04/igem-hokkaidou-logo-transparent.png?w=240&h=240#038;h=240" width="240" height="240" />
-
        background: linear-gradient(top, rgba(0,0,0,0.65) 0%,rgba(0,0,0,0) 100%);
+
         <span class="hokkaidou-description">iGEM HokkaidoU_Japan Official Team Logo</span>
-
        height:100px;
+
    </div>
-
         opacity:0.7;
+
    <div id="hokkaidou-links">
-
         padding:10px;
+
         <ul>
-
      }
+
            <li id="hokkaidou-links-email"><a href="mailto:igemhokkaidou@gmail.com">Email</a></li>
-
      .lof-main-item-desc p{
+
            <li id="hokkaidou-links-facebook"><a href="http://www.facebook.com/iGEMHokkaidoU">Facebook</a></li>
-
         margin:0 8px;
+
            <li id="hokkaidou-links-twitter"><a href="http://twitter.com/iGEM_HokkaidoU">Twitter</a></li>
-
        padding:8px 0
+
            <li id="hokkaidou-links-wordpress"><a href="http://igemhokkaidou.com">WordPress</a></li>
-
      }
+
            <li id="hokkaidou-links-youtube"><a href="http://www.youtube.com/user/iGEMHokkaidoU">YouTube</a></li>
-
      .lof-main-item-desc h3{
+
         </ul>
-
        padding:0;
+
    </div>
-
         margin:0
+
</html>
-
      }
+
{{Team:HokkaidoU_Japan/SocialButtons}}
-
      .lof-main-item-desc h2{
+
<html>
-
         padding:0;
+
</div>
-
        margin:15px 0 0 0px;
+
-
      }
+
-
      .lof-main-item-desc h3 a{ 
+
-
        margin:0;
+
-
        background:#C01F25;
+
-
        font-size:75%;
+
-
        padding:2px 3px;
+
-
        font-family:"Trebuchet MS",Trebuchet,Arial,Verdana,sans-serif;
+
-
        text-transform:uppercase;
+
-
        text-decoration:none
+
-
      }
+
-
      .lof-main-item-desc h3 a:hover{
+
-
         text-decoration:underline;
+
-
      }
+
-
      .lof-main-item-desc h3 i {
+
-
        font-size:70%;
+
-
      }
+
-
      /* main flash */
+
<div id="hokkaidou-right-content">
-
      ul.lof-main-wapper{
+
    <div id="lofslidecontent45" class="lof-slidecontent">
-
         /* margin-right:auto; */
+
        <div style="display: none;" class="preload"><div></div></div>
-
        overflow:hidden;
+
            <div class="lof-main-outer" style="width:900px; height:340px;">
-
        background:transparent url(../images/load-indicator.gif) no-repeat scroll 50% 50%;
+
            <ul style="left: -4200px; width: 6300px;" class="lof-main-wapper">
-
        padding:0px;
+
                <li>
-
        margin:0;
+
                    <img src="https://static.igem.org/mediawiki/2011/6/64/IGEM_HokkaidoU_Index_Slideshow_7.jpg">         
-
        position:absolute;
+
                    <div class="lof-main-item-desc">
-
         overflow:hidden;
+
                        <h2>HokkaidoU_Japan 2011</h2>
-
      }
+
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/8/8a/IGEM_HokkaidoU_Index_Slideshow_1.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Welcome to the BIOxART Gallery!!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/b/b5/IGEM_HokkaidoU_Index_Slideshow_2.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Be careful not to contaminate...</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/0/01/IGEM_HokkaidoU_Index_Slideshow_3.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Meeting, meeting and MEETING!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/d/d1/IGEM_HokkaidoU_Index_Slideshow_4.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Dr. <i>E. coli</i> get ready for the job</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/0/06/IGEM_HokkaidoU_Index_Slideshow_5.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>BIOxART, please feel free to look around</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/7/74/IGEM_HokkaidoU_Index_Slideshow_6.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Experiment, experiment and EXPERIMENT!</h2>
 +
                    </div>
 +
                </li>
 +
            </ul>   
 +
         </div>
 +
        <div class="lof-navigator-wapper">
 +
            <div style="width: 320px; height: 32px;" class="lof-navigator-outer">
 +
                <ul style="width: 54px; left: -160px;" class="lof-navigator">
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/8/81/IGEM_HokkaidoU_Index_Slideshow_Thumb_7.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/f3/IGEM_HokkaidoU_Index_Slideshow_Thumb_1.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/a/aa/IGEM_HokkaidoU_Index_Slideshow_Thumb_2.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/fb/IGEM_HokkaidoU_Index_Slideshow_Thumb_3.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/e/e2/IGEM_HokkaidoU_Index_Slideshow_Thumb_4.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/9/93/IGEM_HokkaidoU_Index_Slideshow_Thumb_5.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/2/21/IGEM_HokkaidoU_Index_Slideshow_Thumb_6.jpg"></li>
 +
                </ul>
 +
            </div>
 +
         </div>
 +
    </div>
 +
    <h2>Abstract</h2>
 +
    <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
 +
    <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
 +
    <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
-
      ul.lof-main-wapper li{
+
    <h2>BioArt</h2>
-
        overflow:hidden;
+
    <iframe id="hokkaidou-bioart-youtube" width="680" height="360" src="http://www.youtube.com/embed/nOP0YIUSj-s" frameborder="0" allowfullscreen></iframe>
-
        padding:0px;
+
    <style type="text/css">
-
        margin:0px;
+
         iframe#hokkaidou-bioart-youtube{ margin-bottom: 30px; }
-
        float:left;
+
-
        position:relative;
+
-
      }
+
-
      .lof-opacity  li{
+
-
        position:absolute;
+
-
        top:0;
+
-
        left:0;
+
-
        float:inherit;
+
-
      }
+
-
      ul.lof-main-wapper li img{
+
-
        padding:0px; 
+
-
      }
+
-
 
+
-
      /* item navigator */
+
-
      .lof-navigator-wapper {
+
-
        position:absolute;
+
-
        bottom:10px;
+
-
        right:10px;
+
-
        background:url(../images/transparent_bg.png) repeat;
+
-
        padding:5px 0px;
+
-
      }
+
-
      .lof-navigator-outer{
+
-
        position:relative;
+
-
        z-index:100;
+
-
        height:180px;
+
-
        width:310px;
+
-
        overflow:hidden;
+
-
        color:#FFF;
+
-
        float:left
+
-
      }
+
-
      ul.lof-navigator{
+
-
        top:0;
+
-
        padding:0;
+
-
        margin:0;
+
-
        position:absolute;
+
-
        width:100%;
+
-
      }
+
-
      ul.lof-navigator li{
+
-
        cursor:hand;
+
-
        cursor:pointer;
+
-
        list-style:none;
+
-
        padding:0;
+
-
        margin-left:0px;
+
-
        overflow:hidden;
+
-
        float:left;
+
-
        display:block;
+
-
 
+
-
        text-align:center;
+
-
 
+
-
      }
+
-
      ul.lof-navigator li img{
+
-
         border:#666 solid 3px; 
+
-
      }
+
-
      ul.lof-navigator li.active img, ul.lof-navigator li:hover img {
+
-
        border:#A8A8A8 solid 3px;
+
-
      }
+
-
      .lof-navigator-wapper .lof-next,  .lof-navigator-wapper .lof-previous{
+
-
        display:block;
+
-
        width:22px;
+
-
        height:30px;
+
-
        color:#FFF;
+
-
        cursor:pointer;
+
-
 
+
-
      }
+
-
      .lof-navigator-wapper .lof-next {
+
-
        float:left; 
+
-
        text-indent:-999px;
+
-
        margin-right:5px;
+
-
        background:url(../images/arrow-l.png) no-repeat right center;
+
-
      }
+
-
      .lof-navigator-wapper .lof-previous {
+
-
        float:left; 
+
-
        text-indent:-999px;
+
-
        margin-left:5px;
+
-
        background:url(../images/arrow-r.png) no-repeat left center;
+
-
      }
+
     </style>
     </style>
-
<div id="lofslidecontent45" class="lof-slidecontent" style="width:980px; height:340px;">
 
-
<div class="preload"><div></div></div>
 
-
  <div class="lof-main-outer" style="width:980px; height:340px;">
 
-
      <ul class="lof-main-wapper">
 
-
          <li>
 
-
                <img src="images/thumbl_980x340.png" title="Newsflash 2" >         
 
-
                <div class="lof-main-item-desc">
 
-
                <h3><a target="_parent" title="Newsflash 1" href="#Category-1">/ Newsflash 1 /</a> <i> — Monday, February 15, 2010 12:42</i></h3>
 
-
                <h2>Content of Newsflash 1</h2>
 
-
                <p>The one thing about a Web site, it always changes! Joomla! makes it easy to add Articles, content,...
 
-
                <a class="readmore" href="#">Read more </a>
 
-
                </p>
 
-
            </div>
 
-
        </li>
 
-
      <li>
 
-
            <img src="images/thumbl_980x340_002.png" title="Newsflash 1" >         
 
-
              <div class="lof-main-item-desc">
 
-
                <h3><a target="_parent" title="Newsflash 2" href="#Category-2">/ Newsflash 2 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
 
-
                <h2>Content of Newsflash 2</h2>
 
-
                <p>Joomla! makes it easy to launch a Web site of any kind. Whether you want a brochure site or you are...
 
-
                <a class="readmore" href="#">Read more </a>
 
-
                </p>
 
-
            </div>
 
-
        </li>
 
-
      <li>
 
-
            <img src="images/thumbl_980x340_003.png" title="Newsflash 3" >           
 
-
              <div class="lof-main-item-desc">
 
-
                <h3><a target="_parent" title="Newsflash 3" href="#Category-3">/ Newsflash 3 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
 
-
                <h2>Content of Newsflash 3</h2>
 
-
                <p>With a library of thousands of free Extensions, you can add what you need as your site grows. Don't...
 
-
                <a class="readmore" href="#">Read more </a>
 
-
                </p>
 
-
            </div>
 
-
        </li>
 
-
        <li>
 
-
            <img src="images/thumbl_980x340_004.png" title="Newsflash 5" >          
+
    <p>We held the new type science art gallery "Bio Art" at the center of Sapporo city.</p>
-
              <div class="lof-main-item-desc">
+
    <p>This gallery is not only for our team.You can also enjoy Bio Art on this Web page.
-
                <h3><a target="_parent" title="Newsflash 4" href="#Category-4">/ Newsflash 4 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
+
    You can watch more detail in <a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice</a> page.</p>
-
                <h2>Content of Newsflash 4</h2>
+
-
                <p>Joomla! 1.5 - 'Experience the Freedom'!. It has never been easier to create your own dynamic Web...
+
</html>
-
                <a class="readmore" href="#">Read more </a>
+
-
                </p>
+
-
            </div>
+
-
        </li>
+
-
       
+
-
        <li>
+
-
            <img src="images/thumbl_980x340_005.png" title="Newsflash 5" >           
+
==Acknowledgements==
-
              <div class="lof-main-item-desc">
+
===Laboratory and Personnel===
-
                <h3><a target="_parent" title="Newsflash 5" href="#">/ Newsflash 5 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
+
* Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
-
              <h2>Content of Newsflash 5</h2>
+
:: Ken-ichi Yamazaki and Members of Yamazaki Lab
-
                <p>Joomla! 1.5 - 'Experience the Freedom'!. It has never been easier to create your own dynamic Web...
+
* Molecular Biology, Institute for Genetic Medicine, Hokkaido University
-
                <a class="readmore" href="#">Read more </a>
+
:: Hisatoshi Shida
-
                </p>
+
* Nikon Imaging Center
-
            </div>
+
:: Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
-
        </li>
+
* Laboratory of Molecular and Cellular Biophysics, Hokkaido University
-
        <li>
+
:: Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
-
 
+
* Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
-
            <img src="images/thumbl_980x340_006.png" title="Newsflash 5" >           
+
:: Taizo Motomura
-
              <div class="lof-main-item-desc">
+
* Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
-
                <h3><a target="_parent" title="Newsflash 6" href="#">/ Newsflash 6 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
+
::Isao Tanaka
-
                <h2>Content of Newsflash 6</h2>
+
* Research Institute for Electronic Science, Hokkaido University
-
                <p>Joomla! 1.5 - 'Experience the Freedom'!. It has never been easier to create your own dynamic Web...
+
-
                <a class="readmore" href="#">Read more </a>
+
-
                </p>
+
-
            </div>
+
-
        </li>
+
-
        <li>
+
-
            <img src="images/thumbl_980x340_007.png" title="Newsflash 5" >           
+
-
              <div class="lof-main-item-desc">
+
-
                <h3><a target="_parent" title="Newsflash 7" href="#">/ Newsflash 7 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
+
-
                <h2>Content of Newsflash 7</h2>
+
-
                <p>Joomla! 1.5 - 'Experience the Freedom'!. It has never been easier to create your own dynamic Web...
+
-
                <a class="readmore" href="#">Read more </a>
+
-
                </p>
+
-
            </div>
+
-
        </li>
+
-
          <li>
+
-
            <img src="images/thumbl_980x340_008.png" title="Newsflash 8" >           
+
-
              <div class="lof-main-item-desc">
+
-
                <h3><a target="_parent" title="Newsflash 8" href="#">/ Newsflash 8 /</a>    <i> — Monday, February 15, 2010 12:42</i></h3>
+
-
                <h2>Content of Newsflash 8</h2>
+
-
                <p>Joomla! 1.5 - 'Experience the Freedom'!. It has never been easier to create your own dynamic Web...
+
-
                    <a class="readmore" href="#">Read more </a>
+
-
                </p>
+
-
            </div>
+
-
        </li>
+
-
      </ul>     
+
-
  </div>
+
-
<div class="lof-navigator-wapper">
+
-
 
+
-
        <div onclick="return false" href="" class="lof-next">Next</div>
+
-
      <div class="lof-navigator-outer">
+
-
            <ul class="lof-navigator">
+
-
              <li><img src="images/thumbs/thumbl_980x340.png" /></li>
+
-
              <li><img src="images/thumbs/thumbl_980x340_002.png" /></li>
+
-
              <li><img src="images/thumbs/thumbl_980x340_003.png" /></li>
+
-
              <li><img src="images/thumbs/thumbl_980x340_004.png" /></li>   
+
-
              <li><img src="images/thumbs/thumbl_980x340_005.png" /></li>
+
-
              <li><img src="images/thumbs/thumbl_980x340_006.png" /></li>     
+
-
              <li><img src="images/thumbs/thumbl_980x340_007.png" /></li>     
+
-
              <li><img src="images/thumbs/thumbl_980x340_008.png" /></li>             
+
-
            </ul>
+
-
      </div>
+
-
        <div onclick="return false" href="" class="lof-previous">Previous</div>
+
-
</div>
+
-
</div>
+
-
</html>
+
-
== トップページ建設予定地 ==
+
===Academic Institutions===
-
今日から本気出す
+
* Salmonella Genetic Stock Center/University of Calgary
 +
* Graduate School of Environmental Earth Science
 +
* Department of biological science/School of Sciense
 +
* Institute for Genetic Medicine
-
デザインは後でやる
+
===Companies===
 +
* Primary Cell Co., Ltd.
 +
* ECONiXE Co., Ltd.
 +
* Hokuto Kohki Engineering Co,. Ltd.
 +
* COSMO BIO Co., Ltd.
 +
* AMINO UP CHEMICAL Co., Ltd.
 +
* Mendel Workshop Co., Ltd.
 +
</div>
{{Team:HokkaidoU_Japan/footer}}
{{Team:HokkaidoU_Japan/footer}}

Latest revision as of 09:59, 15 December 2011

Contents

  • HokkaidoU_Japan 2011

  • Welcome to the BIOxART Gallery!!

  • Be careful not to contaminate...

  • Meeting, meeting and MEETING!

  • Dr. E. coli get ready for the job

  • BIOxART, please feel free to look around

  • Experiment, experiment and EXPERIMENT!

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

BioArt

We held the new type science art gallery "Bio Art" at the center of Sapporo city.

This gallery is not only for our team.You can also enjoy Bio Art on this Web page. You can watch more detail in Human Practice page.

Acknowledgements

Laboratory and Personnel

  • Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
Ken-ichi Yamazaki and Members of Yamazaki Lab
  • Molecular Biology, Institute for Genetic Medicine, Hokkaido University
Hisatoshi Shida
  • Nikon Imaging Center
Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
  • Laboratory of Molecular and Cellular Biophysics, Hokkaido University
Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
  • Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
Taizo Motomura
  • Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
Isao Tanaka
  • Research Institute for Electronic Science, Hokkaido University

Academic Institutions

  • Salmonella Genetic Stock Center/University of Calgary
  • Graduate School of Environmental Earth Science
  • Department of biological science/School of Sciense
  • Institute for Genetic Medicine

Companies

  • Primary Cell Co., Ltd.
  • ECONiXE Co., Ltd.
  • Hokuto Kohki Engineering Co,. Ltd.
  • COSMO BIO Co., Ltd.
  • AMINO UP CHEMICAL Co., Ltd.
  • Mendel Workshop Co., Ltd.
Retrieved from "http://2011.igem.org/Team:HokkaidoU_Japan"