Team:UT-Tokyo

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=Welcome to iGEM UT-Tokyo=
=Welcome to iGEM UT-Tokyo=
Welcome to the UT-Tokyo team's wiki for iGEM 2011.
Welcome to the UT-Tokyo team's wiki for iGEM 2011.
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This site will be updated as we progress through our project.
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If you would like to get in touch with us, please contact us by [mailto:info@igem-ut.net email] or on [http://twitter.com/UT_Tokyo twitter (@UT_Tokyo)], [http://www.facebook.com/pages/IGEM-UT-Tokyo/246851982004135 Facebook].
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If you would like to get in touch with us, please contact us by [mailto:info@igem-ut.net email] or on [http://twitter.com/UT_Tokyo twitter (@UT_Tokyo)].
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=Our Project: SMART ''E.coli'' <html><span style="font-size: 50%">- Self Mustering with Aspartate-Responsive Taxis -</span></html>=
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==Making bioremediation far more efficient!==
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=Project Overview (as of 2011/09/02)=
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Our project, SMART ''E.coli'', aims at making bioremediation more efficient. In situations where the substrate (such as pollutants you want bacteria to collect) is not equally distributed and rather concentrated in particular spots, conventional bioremediation projects are not efficient. To overcome this problem, we attempt to increase the cell density around the substrate by attracting the bacteria to the substrate.
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Our team project is the creation of "''E. Coli'' attracted to stress."
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This project consists of two parts: detection of stress and "positive stressotaxis." To detect stress, we use the promoters of the SOS genes, coding for proteins involved in well-known DNA-repair mechanisms. There are various SOS genes in ''E. coli'' and therefore we think it is possible to detect various ranges of stress using different promoters. In addition, combining stress-responsive promoters with chemotactic factors, we aim to create ''E. coli'' that swim towards the source of the stress.
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To attain this goal, we designed two mechanisms, chemoattraction using aspartate and cell arrest.
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We propose that the resulting machine possessing these functions may be useful for applications such as bioremediation and environmental screening.
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To learn more about our project, please take a look at [[Team:UT-Tokyo/Project|our Project page]] on this wiki!
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Bio-systems as a means for environmental remediation have been extensively investigated. However, these devices have often been limited by the requirement of a high cell density at the target site in order to achieve higher efficiencies.
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To overcome this, we devise an inducible self-assembling system in Escherichia coli utilizing L-aspartate (L-Asp)chemotaxis.
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Our system consists of two cell types: “guiders” and “workers”. When exposed to a signal, the former discharge and generate a signal-centered spatial L-Asp gradient, and the latter lose motility by repression of a flagellum-regulating gene (cheZ).These cells remain at the source of the signal and cannot escape.
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Using E. coli-derived stress-sensitive promoters cloned de novo, we provide evidence that our system enables auto-assembly and localization after exposure to ultraviolet radiation.
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Since the input can be varied to other inducible promoters, we anticipate that our system to greatly enhance the potential of engineered cellular machineries.
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[[File:UT-Tokyo_Overview.png]]
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Latest revision as of 01:24, 6 October 2011

Welcome to iGEM UT-Tokyo

Welcome to the UT-Tokyo team's wiki for iGEM 2011. If you would like to get in touch with us, please contact us by email or on [http://twitter.com/UT_Tokyo twitter (@UT_Tokyo)], [http://www.facebook.com/pages/IGEM-UT-Tokyo/246851982004135 Facebook].

Our Project: SMART E.coli - Self Mustering with Aspartate-Responsive Taxis -

Making bioremediation far more efficient!

Our project, SMART E.coli, aims at making bioremediation more efficient. In situations where the substrate (such as pollutants you want bacteria to collect) is not equally distributed and rather concentrated in particular spots, conventional bioremediation projects are not efficient. To overcome this problem, we attempt to increase the cell density around the substrate by attracting the bacteria to the substrate.

To attain this goal, we designed two mechanisms, chemoattraction using aspartate and cell arrest.

To learn more about our project, please take a look at our Project page on this wiki!

UT-Tokyo Overview.png