Team:Tokyo Metropolitan
From 2011.igem.org
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</html><html><div align="center"> | </html><html><div align="center"> | ||
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<tr height="40" border="0"> | <tr height="40" border="0"> | ||
- | <td width="94" border="0"><div class="rollover01"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan" border="0"><img src=" | + | <td width="94" border="0"><div class="rollover01"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan" border="0"><img src="https://static.igem.org/mediawiki/2011/5/56/Tokyo_Metropolitan_home.png"></div></a></td> |
- | <td width="88"><div class="rollover02"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Team" border="0"><img src=" | + | <td width="88"><div class="rollover02"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Team" border="0"><img src="https://static.igem.org/mediawiki/2011/f/ff/Tokyo_Metropolitan_team.png"></div></a></td> |
- | <td width="108"><div class="rollover03"><a href="https://igem.org/Team.cgi?year=2011&team_name=Tokyo_Metropolitan" border="0"><img src=" | + | <td width="108"><div class="rollover03"><a href="https://igem.org/Team.cgi?year=2011&team_name=Tokyo_Metropolitan" border="0"><img src="https://static.igem.org/mediawiki/2011/0/06/Tokyo_Metropolitan_profile.png"></div></a></td> |
- | <td width="123"><div class="rollover04"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project" border="0"><img src=" | + | <td width="123"><div class="rollover04"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project" border="0"><img src="https://static.igem.org/mediawiki/2011/9/99/Tokyo_Metropolitan_project.png"></div></a></td> |
- | <td width="83"><div class="rollover05"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Parts" border="0"><img src=" | + | <td width="83"><div class="rollover05"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Parts" border="0"><img src="https://static.igem.org/mediawiki/2011/5/53/Tokyo_Metropolitan_parts.png"></div></a></td> |
- | <td width="163"><div class="rollover06"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Notebook" border="0"><img src=" | + | <td width="163"><div class="rollover06"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Notebook" border="0"><img src="https://static.igem.org/mediawiki/2011/8/8b/Tokyo_Metropolitan_notebook.png"></div></a></td> |
- | <td width="118"><div class="rollover07"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Safety" border="0"><img src=" | + | <td width="118"><div class="rollover07"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Safety" border="0"><img src="https://static.igem.org/mediawiki/2011/a/a4/Tokyo_Metropolitan_safety.png"></div></a></td> |
- | <td width="198"><div class="rollover08"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Attributions" border="0"><img src=" | + | <td width="198"><div class="rollover08"><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Attributions" border="0"><img src="https://static.igem.org/mediawiki/2011/5/5d/Tokyo_Metropolitan_attributions.png"></div></a></td> |
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<tr height="80"><td width="950" colspan="3"> | <tr height="80"><td width="950" colspan="3"> | ||
- | <table align="center"><tr><td width=" | + | <table align="center"><tr><td width="650" align="left"> |
- | <font size="4">Welcome!</font | + | <font size="4">Welcome!</font><hr> |
- | + | This is team Tokyo_Metropolitan 2011 wiki. We have a project "BeE.coli" on iGEM 2011 and tackled safety issues or human practice proposal. Please click menu to see more our pages! | |
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</td> | </td> | ||
<td align="center" valign="bottom"><a href="http://twitter.com/#!/igemtmu"> | <td align="center" valign="bottom"><a href="http://twitter.com/#!/igemtmu"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/2011/d/d5/Tokyo_Metropolitan_twitter.png" height="80"> |
<br><font size="1">Follow us on twitter!</font></a></td> | <br><font size="1">Follow us on twitter!</font></a></td> | ||
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<td align="center" valign="bottom"><a href="https://2011.igem.org/Main_Page"> | <td align="center" valign="bottom"><a href="https://2011.igem.org/Main_Page"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/2011/6/62/Tokyo_Metropolitan_igemlogo.png" height="80"> |
<br><font size="1">Go to iGEM 2011 Main Page</font></a></td> | <br><font size="1">Go to iGEM 2011 Main Page</font></a></td> | ||
</tr></table> | </tr></table> | ||
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<td width="30"></td> | <td width="30"></td> | ||
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<font size="4">Abstract : BeE.coli</font> | <font size="4">Abstract : BeE.coli</font> | ||
<hr> | <hr> | ||
- | + | Team Tokyo_Metropolitan 2011 propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.<br> | |
- | + | To accomplish this project, we planned to add three functions to E.coli. First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation.<br> | |
- | + | Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria.<br> | |
- | + | Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene.<br> | |
- | + | In the future, BeE.coli will be useful for medical scenes. | |
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</td> | </td> | ||
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<table border="0"><tr height="83"><td width="385"></td><td width="220"> | <table border="0"><tr height="83"><td width="385"></td><td width="220"> | ||
<div align="center"> | <div align="center"> | ||
Taxis for AHL!<br> | Taxis for AHL!<br> | ||
BeE.coli go to area where target bacteria is concentrated.<br> | BeE.coli go to area where target bacteria is concentrated.<br> | ||
- | + | <a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Targeting">⇒See detail.</a> | |
</div> | </div> | ||
</td><td width="45"></td></tr></table> | </td><td width="45"></td></tr></table> | ||
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<table border="0"><tr height="83"><td width="440"></td><td width="210"> | <table border="0"><tr height="83"><td width="440"></td><td width="210"> | ||
<div align="center"> | <div align="center"> | ||
- | + | Get high speed move!<br> | |
BeE.coli have mutant HNS protein(T108I).<br> | BeE.coli have mutant HNS protein(T108I).<br> | ||
- | + | <a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/SpeedUp">⇒See details</a> | |
</div> | </div> | ||
</td></tr></table> | </td></tr></table> | ||
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<table border="0"><tr height="83"><td width="325"></td><td width="220"> | <table border="0"><tr height="83"><td width="325"></td><td width="220"> | ||
<div align="center"> | <div align="center"> | ||
Sting pathogens!<br> | Sting pathogens!<br> | ||
BeE.coli send holin gene to target bacteria with conjugation.<br> | BeE.coli send holin gene to target bacteria with conjugation.<br> | ||
- | + | <a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Killing">⇒See detail</a> | |
</div> | </div> | ||
</td><td width="105"></td></tr></table> | </td><td width="105"></td></tr></table> | ||
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</td></tr> | </td></tr> | ||
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</table> | </table> | ||
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</tr> | </tr> | ||
- | <tr height=" | + | <tr height="20"><td width="950" colspan="3" align="center"><hr></td></tr> |
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+ | <table><tr><td width="450" align="center"><b>Photo Gallery</b><br><embed type="application/x-shockwave-flash" src="https://picasaweb.google.com/s/c/bin/slideshow.swf" width="400" height="267" flashvars="host=picasaweb.google.com&hl=en_US&feat=flashalbum&RGB=0x000000&feed=https%3A%2F%2Fpicasaweb.google.com%2Fdata%2Ffeed%2Fapi%2Fuser%2FigemTMU%2Falbumid%2F5659929053369567921%3Falt%3Drss%26kind%3Dphoto%26hl%3Den_US" pluginspage="http://www.macromedia.com/go/getflashplayer"></embed></td><td width="450" align="center"><b>Our creation! Safety Promotion Video</b><br><br><iframe width="400" height="267" src="http://www.youtube.com/embed/JZwjYAyRaWk?hl=ja&fs=1" frameborder="0" allowfullscreen></iframe><Br><a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Safety">⇒Click to see safety page.</a></td></tr><table> | ||
Latest revision as of 21:18, 5 October 2011
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Abstract : BeE.coli
Team Tokyo_Metropolitan 2011 propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed. To accomplish this project, we planned to add three functions to E.coli. First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation. Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria. Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene. In the future, BeE.coli will be useful for medical scenes. |
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Photo Gallery | Our creation! Safety Promotion Video ⇒Click to see safety page. |