Team:USC/Notebook/Week13

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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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[[Team:USC/Notebook/Brainstorming|Brainstorming]]
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[[Team:USC/Notebook/Week13|Week 13]]
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[[Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 13:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 13:'''</h3>

Latest revision as of 03:58, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 13:

09/06/2011
1. Digest/ ligate casO+PCDF
2. Transform ligated products into BL21 with tetO::GFP
3. Transform CRISPR GFP#7 into BL21 with tetO::GFP
4. Inoculate HT5 with/without IPTG
5. Inoculate HT115+tetO::GFP
6. Purify casO (PCR products)
7. Measure the DNA of casO::PCDF


09/07/2011
1. Make competent cells as follows:
BL21 (tetO::GFP, CRISPR::GFP)
HT115(tetO::GFP, CRISPR::GFP)
HT115(tetO::GFP)
2. Take OD reading of HT115 with/ without IPTG
3. Digest/ligate casO+PCDF
4. Transform CRISPR#7 into tetO cells(BL21)
5. Take equal amount of E.Coli, miniprep, transform into new DH5α, select on amp.


09/19/2011
1. Growth test, grow all the bacteria overnight

2. PCR cas3+casO from gDNA


09/08/2011
1. OD reading
2. Digest/ligate reaction
tetR with pSB1k3
tetO with pSB1k3
casO with PCDF
Transform into DH5α, and also IPTG samples into DH5α

09/09/2011
1. Make BL21(tetO, CRISPR-GFP #7) competent and make freeze stock
2. Make HT115(tetO, CRISPR-GFP #7) competent and make freeze stock
3. Make HT115(CRISPR-GFP #7) competent and make freeze stock

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week13"