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Team:Arizona State/Notebook/September
From 2011.igem.org
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:* Listeria CRISPR only uses cas9 tracr gene | :* Listeria CRISPR only uses cas9 tracr gene | ||
:* Project requires: | :* Project requires: | ||
| - | ::* | + | ::* Cas9 |
::* Duet plasmid | ::* Duet plasmid | ||
::* Listeria T7 RSR | ::* Listeria T7 RSR | ||
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::* some distortion and low resolution | ::* some distortion and low resolution | ||
== Sunday, September 4 to Friday, September 9 == | == Sunday, September 4 to Friday, September 9 == | ||
| - | * | + | * Cas9 PCR products restricted overnight |
== Saturday, September 10 == | == Saturday, September 10 == | ||
* 9/10 restriction products dephosphorylated for 2 hours | * 9/10 restriction products dephosphorylated for 2 hours | ||
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* 9/8 restriction - 9/10 ligation products transformed into NEB cells | * 9/8 restriction - 9/10 ligation products transformed into NEB cells | ||
== Sunday, September 18 to Saturday, September 24 == | == Sunday, September 18 to Saturday, September 24 == | ||
| - | * | + | *Attempt to assemble CMR-RSR using overlapping oligos |
| + | *Began preparation of pSB1C3 plasmid for BioBrick preparation | ||
== Sunday, September 25 to Wednesday, September 28 == | == Sunday, September 25 to Wednesday, September 28 == | ||
| - | * | + | *Finalized BioBrick preparation for intended BioBrick part submission |
| + | *Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run. | ||
| + | *Wiki parties all week in the lab! | ||
| + | *Submitted BioBricks! | ||
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Latest revision as of 03:09, 29 September 2011
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 Thursday, September 1
Saturday, September 3
Sunday, September 4 to Friday, September 9
Saturday, September 10
Sunday, September 11 to Saturday, September 17
Sunday, September 18 to Saturday, September 24
Sunday, September 25 to Wednesday, September 28
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