Team:UT Dallas/week12
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9/19/11</td><td valign='top'> Full Digestion/Electrophoresis: CheY, CheZ*, pSB1C3<br> | 9/19/11</td><td valign='top'> Full Digestion/Electrophoresis: CheY, CheZ*, pSB1C3<br> | ||
PCR/Gel Electrophoresis: CheY<br> | PCR/Gel Electrophoresis: CheY<br> | ||
- | Ligation: ctx + CheZ*, ctx + CheY, pYeaR-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3<br> | + | Ligation:<br> ctx + CheZ*, ctx + CheY, pYeaR-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3<br> |
- | Incubation: scp-ToxR-FGFR, ToxR'MalE/ZIP, ToxR'MalE | + | Incubation:<br> scp-ToxR-FGFR, ToxR'MalE/ZIP, ToxR'MalE |
</td></tr><tr><td valign='top'> </td><td valign='top'> </td></tr><tr><td valign='top'> | </td></tr><tr><td valign='top'> </td><td valign='top'> </td></tr><tr><td valign='top'> | ||
9/20/11</td><td valign='top'> PCR/Gel Purify: ctx<br> | 9/20/11</td><td valign='top'> PCR/Gel Purify: ctx<br> |
Latest revision as of 06:25, 28 September 2011
Week 12
9/18/11 | Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY Test Digestion: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY |
9/19/11 | Full Digestion/Electrophoresis: CheY, CheZ*, pSB1C3 PCR/Gel Electrophoresis: CheY |
9/20/11 | PCR/Gel Purify: ctx Maltose Induction Experiment: DATA COLLECTION |
9/21/11 | PCR: ctx (retry) Test Digestion/Purification: pYeaR-ToxR-FGFR |
9/22/11 | Miniprep/Glycerol Stock: ctx-GFP, ctx-CheZ, ctx-CheY Full Digestion: pSB1C3 |
9/23/11 | Miniprep: psct-RBS-LuxI-term Dissolving/Diluting Primers: Germany Parts |
9/24/11 | Dilution 1:10 for Optical Density readings: scp-ToxR-FGFR, pYeaR-ToxR-FGFR Growing from glycerol stock (backup): scp-ToxR-FGFR, pYeaR-ToxR-FGFR ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S |
9/25/11 | Dilution 1:20 for experimentation: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S Running Maltose Induction Experiment: DATA COLLECTION (fluorescence) |
9/26/11 | Dilution 1:5 for experimentation: pYear-ToxR-FGFR, scp-ToxR-FGFR Heparin/FGF Induction Experiment (re-run): DATA COLLECTION (fluorescence) |