Team:UT Dallas/week10

From 2011.igem.org

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(Created page with "<h2>Week 10</h2> <table> <tr><td valign='top'>9/11/11</td><td valign='top'>Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ, </td></tr><tr><td valign='top'>&nb...")
 
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<h2>Week 10</h2>
<h2>Week 10</h2>
<table>
<table>
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<tr><td valign='top'>9/11/11</td><td valign='top'>Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,  
+
<tr><td valign='top'>9/6/11</td><td valign='top'>Incubation:<br>Germany Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] (FHK12)<br>
 +
  CheY, PyeaR-GFP-RBS-TetR-term, scp-GFP-RBS-TetR-Term<br>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
9/12/11 Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,<br>
+
<tr><td valign='top'>9/7/11</td><td valign='top'> Glycerol Stock/Miniprep: [pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN]<br>
-
Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
+
Incubation: pYeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-Tetr-Term, AHL inducible Colicin, Pcst-RBS-LuxI-Term<br>
-
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
+
Full Digestion/Gel Electrophoresis+Purification: FGFR<br>
-
Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP<br>
+
Ligation 10 Min: Vectors: FGFR, Inserts; ToxR<br>
-
Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP<br>
+
Transformation: ToxR-FGFR
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Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx<br>
+
-
Incubation: CheZ* (Chlora)
+
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
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<tr><td valign='top'>9/13/11</td><td valign='top'>Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR<br>
+
<tr><td valign='top'>9/8/11</td><td valign='top'> Dilution of Pcst-RBS-:uxI-Term, AHL induible colicin<br>
-
Full Digestion: ToxR-FGFR (at E/X as vector)<br>
+
Incubation: DATA COLLECTION=GFP quantification [(a) Psct-RBS-LuxI-Term vs. (b) AHL inducible colicin](DH5-alpha) <br>
-
Ligation: Vector-> ToxR-FGFR  Insert-> ctx<br>
+
Glycerol Stock/Miniprep: Pyear-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term<br>
-
Incubation: CheY
+
Test Digestion: Pyear-GFP-RBS-Term, scp-GFP-RBS-TetR-Term<br>
 +
Colony PCR/ Gel Ectrophoresis: Toxr-FGFR
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>9/14/11</td><td valign='top'>Transformation: ctx-ToxR-FGFR<br>
+
<tr><td valign='top'>9/9/11</td><td valign='top'> Full Digestion: FGFR, RBS-GFP-TetR-Term, CheY, CheZ*, ctx-CheZ<br>
-
Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S<br>
+
Test Digestion: Pyear-GFp-RBS-TetR-Term, scp-GFP-RBS-TetR-Term<br>
-
Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb)<br>
+
Ligation 10 Min: Vectors: CheY, CheZ*, FGFR, RBS-GFP  Inserts: Ctx<br>
-
Miniprep: CheY<br>
+
Transformation: FGFR, ToxR-FGFR, ctx-CheZ*-RBS-GFP, CheZ*, ctx-CheZ*<br>
-
PCR: CheY<br>
+
-
Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*
+
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-
<tr><td valign='top'>9/15/11</td><td valign='top'>PCR: CheY<br>
+
<tr><td valign='top'>9/10/11</td><td valign='top'> Colony PCR: ToxR-FGFR, ctx-CheZ*, ctx-CheZ </td></tr>
-
Full Digestion: CheY (X/S), vector pSB1C3<br>
+
-
Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP<br>
+
-
Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP<br>
+
-
Dephosphorylation: Vector pSB1C3<br>
+
-
Ligation: CheY + pSB1c3<br>
+
-
Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ*, ctx-CheZ-RBS-GFP, ctx<br>
+
-
Ligation: ctx + CheZ*<br>
+
-
Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey
+
-
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
+
-
<tr><td valign='top'>9/16/11</td><td valign='top'>Digestion: ctx-CheZ<br>
+
-
Incubation: ToxR'MalE/ZIP, ToxR'MalE, <br>
+
-
Ligation: ctx + CheY (retry)<br>
+
-
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
+
-
<tr><td valign='top'>9/17/11</td><td valign='top'>Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY</td></tr>
+
</table>
</table>

Latest revision as of 00:54, 28 September 2011

Week 10

9/6/11Incubation:
Germany Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] (FHK12)

CheY, PyeaR-GFP-RBS-TetR-term, scp-GFP-RBS-TetR-Term

  
9/7/11 Glycerol Stock/Miniprep: [pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN]

Incubation: pYeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-Tetr-Term, AHL inducible Colicin, Pcst-RBS-LuxI-Term
Full Digestion/Gel Electrophoresis+Purification: FGFR
Ligation 10 Min: Vectors: FGFR, Inserts; ToxR
Transformation: ToxR-FGFR

  
9/8/11 Dilution of Pcst-RBS-:uxI-Term, AHL induible colicin

Incubation: DATA COLLECTION=GFP quantification [(a) Psct-RBS-LuxI-Term vs. (b) AHL inducible colicin](DH5-alpha)
Glycerol Stock/Miniprep: Pyear-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term
Test Digestion: Pyear-GFP-RBS-Term, scp-GFP-RBS-TetR-Term
Colony PCR/ Gel Ectrophoresis: Toxr-FGFR

  
9/9/11 Full Digestion: FGFR, RBS-GFP-TetR-Term, CheY, CheZ*, ctx-CheZ

Test Digestion: Pyear-GFp-RBS-TetR-Term, scp-GFP-RBS-TetR-Term
Ligation 10 Min: Vectors: CheY, CheZ*, FGFR, RBS-GFP Inserts: Ctx
Transformation: FGFR, ToxR-FGFR, ctx-CheZ*-RBS-GFP, CheZ*, ctx-CheZ*

  
9/10/11 Colony PCR: ToxR-FGFR, ctx-CheZ*, ctx-CheZ
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