Team:USC/Notebook/Week9

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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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[[Team:USC/Notebook/Brainstorming|Brainstorming]]
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
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[[Team:USC/Notebook/Week1|Week 1]]
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[[Team:USC/Notebook/Week2|Week 2]]
[[Team:USC/Notebook/Week2|Week 2]]
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[[Team:USC/Notebook/Week5|Week 5]]
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[[Team:USC/Notebook/Week9|Week 9]]
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[[Team:USC/Notebook/Week10|Week 10]]
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[[Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3>
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Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later
Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later
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Latest revision as of 03:57, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 9:

08/01/2011
1. Lab meeting: set up all the work for the week
2. PCR CAS3
3. Make competent cells of tetR+tetO, grow overnight
4. Run digestion for PCOLA::CASO (7 samples)


08/02/2011
1. Mini prep DNA isolation of 23 plasmid
2. Digestion by BamHI and NcoI
3. Run a gel to verify
4. Prepared samples for DNA sequencing, including old ones from 07/28/2011
5. Mini-prep 48 colonies from PCOLA with CASO+4 colonies from PCDF with CASO
6. Digest 10 µL of miniprep for verification by gel


08/03/2011
1. Repeat 8 mini-prep from PCOLA with CASO
2. Digest samples from 6-8
3. PCR PCDF+CASO#4 to check if this plasmid has CASO or not
Have a positive control using 1mL of DH5α gDNA


08/04/2011
1. Make competent cells for tetR and tetO
2. Digest POCLA by NcoI-HF
3. PCR PCDF+CASO#4
Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week9"