Team:USC/Notebook/Week7

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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 7:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 7:'''</h3>
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3. Test the digested tetR by running a gel
3. Test the digested tetR by running a gel
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Latest revision as of 03:57, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 7:

07/19/2011
1. Gel verification for CASO (1-8)
Observation: in second row, GFP spcer, tetR spacer, CRISPR#1, and CRISPR #2
2. Gel verification by digestion
3. Purify tetR from Gel
4. Ligate pSB1C3 with tetR, place in the 15c incubator overnight
5. Transformation


07/21/2011
1. Transform ligations of tetR in DH5α and top10 competent cells
2. Inoculate PCOLA, PCDF and CRISPR


07/22/2011
1. Mini-prep for :
tetR in DH5α and top10 (16 samples)
CRISPR (2 samples)
PCOLA (1 sample)
PCDF (5 samples)
2. Digest tetR with EcoR1 and Pst1
3. Test the digested tetR by running a gel

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week7"