Team:USC/Notebook/Week5

From 2011.igem.org

(Difference between revisions)
(Created page with "<table> <!-- usc&igem logo --> <tr> <td> <table> <tr> <td style="width:330px;">File:USC-logo.jpg</td> <td style="width:504px;">File:IGEM2011-logo.jpg</td> <td style="wid...")
 
(3 intermediate revisions not shown)
Line 3: Line 3:
<tr>
<tr>
-
<td>
+
<td colspan="3">[[File:USC_Banner.jpg]]</td>
-
<table>
+
-
<tr>
+
-
<td style="width:330px;">[[File:USC-logo.jpg]]</td>
+
-
<td style="width:504px;">[[File:IGEM2011-logo.jpg]]</td>
+
-
<td style="width:150px;">[[File:IGEM-logo.jpg]]</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td>[[File:USC_Banner.jpg]]</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td  colspan="2">
+
<td  colspan="3">
<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;">
<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;">
<ul>
<ul>
-
<span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span>
+
<span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span>
+
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span>
+
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span>
-
                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span>
+
                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span>
+
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span>
+
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span>
+
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span>
-
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
+
                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span>
 +
                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
</ul>
</ul>
 +
</div>
</div>
 +
 +
</td>
</td>
</tr>
</tr>
<tr>
<tr>
-
<td>
+
<td style="width:100px; vertical-align:top;">
-
<!-- Original content -->
+
 
<table>
<table>
<tr>
<tr>
 +
<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
 +
</tr>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
<br/>
<br/>
-
[[File:Brain_storm.JPG]]
+
[[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 1]]
+
[[Team:USC/Notebook/Week1|Week 1]]
<br/>
<br/>
-
[[File:week1.jpg]]
+
[[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]]
</td>
</td>
-
 
+
<td style="text-align:center; font-family:Verdana; font-weight:700;">
 +
[[Team:USC/Notebook/Week2|Week 2]]
 +
<br/>
 +
[[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]]
 +
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 2]]
+
[[Team:USC/Notebook/Week3|Week 3]]
<br/>
<br/>
-
[[File:week2.jpg]]
+
[[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 3]]
+
[[Team:USC/Notebook/Week4|Week 4]]
<br/>
<br/>
-
[[File:week3.jpg]]
+
[[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]]
</td>
</td>
 +
 +
<tr>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 4]]
+
[[Team:USC/Notebook/Week5|Week 5]]
<br/>
<br/>
-
[[File:week4.jpg]]
+
[[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 5]]
+
[[Team:USC/Notebook/Week6|Week 6]]
<br/>
<br/>
-
[[File:week5.jpg]]
+
[[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 6]]
+
[[Team:USC/Notebook/Week7|Week 7]]
<br/>
<br/>
-
[[File:week6.jpg]]
+
[[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]]
</td>
</td>
-
 
-
<tr>
 
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 7]]
+
[[Team:USC/Notebook/Week8|Week 8]]
<br/>
<br/>
-
[[File:week7.jpg]]
+
[[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 8]]
+
[[Team:USC/Notebook/Week9|Week 9]]
<br/>
<br/>
-
[[File:week8.jpg]]
+
[[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]]
</td>
</td>
 +
 +
<tr>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 9]]
+
[[Team:USC/Notebook/Week10|Week 10]]
<br/>
<br/>
-
[[File:week9.jpg]]
+
[[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 10]]
+
[[Team:USC/Notebook/Week11|Week 11]]
<br/>
<br/>
-
[[File:week10.jpg]]
+
[[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 11]]
+
[[Team:USC/Notebook/Week12|Week 12]]
<br/>
<br/>
-
[[File:week11.jpg]]
+
[[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 12]]
+
[[Team:USC/Notebook/Week13|Week 13]]
<br/>
<br/>
-
[[File:week12.jpg]]
+
[[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]]
</td>
</td>
<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
-
[[Team:USC/Notebook/Brainstorming|Week 13]]
+
[[Team:USC/Notebook/Week14|Week 14]]
<br/>
<br/>
-
[[File:week13.jpg]]
+
[[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]]
 +
</td>
 +
 
 +
</tr>
 +
</table>
</td>
</td>
</tr>
</tr>
-
<tr>
+
</table>
-
<td colspan="7">
+
 
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 5:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 5:'''</h3>
Line 167: Line 171:
5. PCR CASO by using old primers
5. PCR CASO by using old primers
<br />
<br />
-
 
-
 
-
</table>
 

Latest revision as of 03:56, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 5:

07/05/2011
1. digestion step by step for MET25 plasmid see protocols in general protocols


07/06/2011
1. Purify Gel DNA
2. Run a gell test :
MET25, pRS423-426, tetO+cFP, tetO+RFP, tetO+GFP,CAS3, CASO 3. PCR CASO primers


07/07/2011
1. Transformation for all 4 ligation plasmids:
TPS1, MSN2, ELO1, OLE1+pRS vectors
2. Gel verify for CASO
3. Amplify the verified products by PCR
4. Reisolate the genomic DNA
5. PCR CASO by using old primers

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week5"