Team:USC/Notebook/Week4

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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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[[Team:USC/Notebook/Brainstorming|Brainstorming]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 4:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 4:'''</h3>
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BBa-I15010
BBa-I15010
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<span style="font-family:Verdana; font-weight:700;"> 06/30/2011 </span>
<span style="font-family:Verdana; font-weight:700;"> 06/30/2011 </span>
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2. Transform and inoculate tetR, tetO, CASO and CRISPR
2. Transform and inoculate tetR, tetO, CASO and CRISPR
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Observation: tetO didn’t work, OLE1 and MSN2 worked
Observation: tetO didn’t work, OLE1 and MSN2 worked
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Latest revision as of 03:56, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 4:

06/27/2011
1. Lab meeting


06/28/2011
1. Observation from yesterday:
Only transformation of OLE1grow on plates, others don’t
2. PCR MSN ligation products in Cyle #1, pick E, F, G , H colony
3. Ligate OLE1, MSN2, TSP1, ELO1 with pRS vectors 423-426
4. Transform the ligation samples
5. Plasmid DNA purification for:
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010


06/30/2011
1. Digest MET25 and pRS vectors
Notes: for pRS vectors, add 1 µL of phosphatase, incubate for 30min and spin column purify
For MET25 promoter, run reaction on a1% agarose gel, then cut out the agarose and purify with column
2. Transform and inoculate tetR, tetO, CASO and CRISPR


07/01/2011
1. Mini-prep
tetR, MET25, CRISPR and tetO
2. Gel verification for
TPS1, MSN2, ELO1, OLE1, tetO, MET25, CRISPR, tetR
Observation: tetO didn’t work, OLE1 and MSN2 worked

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