Team:USC/Notebook/Week1

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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span>
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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
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                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span>
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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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[[Team:USC/Notebook/Brainstorming|Brainstorming]]
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
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[[Team:USC/Notebook/Brainstorming|Week 1]]
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[[Team:USC/Notebook/Week1|Week 1]]
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[[Team:USC/Notebook/Week2|Week 2]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 1:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 1:'''</h3>
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F. Plate 4 Well 6O
F. Plate 4 Well 6O
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<span style="font-family:Verdana; font-weight:700;"> 06/09/2011 </span>
<span style="font-family:Verdana; font-weight:700;"> 06/09/2011 </span>
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1. Freeze the inoculated culture
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2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others
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<span style="font-family:Verdana; font-weight:700;"> 06/11/2011 </span>
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1. Purify plasmid DNA from the culture
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Latest revision as of 03:55, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 1:

06/07/2011
Transformation plasmids into DH5α Competent cells
Plasmids are from iGEM plates:
A. Plate 2 Well 7E (AHL signaling)
B. Plate 3 Well 20F (LovTAP composite)
C. Plate 4 Well 16O (LovTAP Composite)
D. Plate 1 Well 2B (GFP+PEST191)
E. Plate 2 Well 4A (Yeast ADH1 promoter)


06/08/2011
Transformation of E. Coli, Plasmids are from iGEM plates:
Plasmids are from iGEM plates:
A. Plate 2 Well 13J
B. Plate 1 Well 20F
C. Plate 4 Well 16M
D. Plate 1 Well 16K
E. Plate 3 Well 14H
F. Plate 4 Well 6O

06/09/2011
1. Inoculation for all the plasmid we have transformed before. Observation: all transformation except BBa-I15010

06/10/2011
1. Freeze the inoculated culture
2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others

06/11/2011
1. Purify plasmid DNA from the culture

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week1"