Team:USC/Notebook/Week1
From 2011.igem.org
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<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | <div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | ||
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- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span> |
- | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span> | |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span> |
+ | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span> | ||
</ul> | </ul> | ||
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</td> | </td> | ||
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<table> | <table> | ||
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+ | <h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1> | ||
+ | </tr> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Brainstorming|Brainstorming]] | [[Team:USC/Notebook/Brainstorming|Brainstorming]] | ||
<br/> | <br/> | ||
- | [[File:Brain_storm.JPG]] | + | [[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week1|Week 1]] |
<br/> | <br/> | ||
- | [[File:week1.jpg]] | + | [[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]] |
</td> | </td> | ||
- | + | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | |
+ | [[Team:USC/Notebook/Week2|Week 2]] | ||
+ | <br/> | ||
+ | [[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]] | ||
+ | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week3|Week 3]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week4|Week 4]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]] |
</td> | </td> | ||
+ | |||
+ | <tr> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week5|Week 5]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week6|Week 6]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week7|Week 7]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]] |
</td> | </td> | ||
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<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week8|Week 8]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week9|Week 9]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]] |
</td> | </td> | ||
+ | |||
+ | <tr> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week10|Week 10]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week11|Week 11]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week12|Week 12]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week13|Week 13]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]] |
</td> | </td> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
- | [[Team:USC/Notebook/ | + | [[Team:USC/Notebook/Week14|Week 14]] |
<br/> | <br/> | ||
- | [[File: | + | [[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]] |
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | </table> | ||
</td> | </td> | ||
</tr> | </tr> | ||
- | < | + | </table> |
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 1:'''</h3> | <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 1:'''</h3> | ||
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F. Plate 4 Well 6O | F. Plate 4 Well 6O | ||
+ | <br /> | ||
- | </ | + | <span style="font-family:Verdana; font-weight:700;"> 06/09/2011 </span> |
+ | <br /> | ||
+ | 1. Inoculation for all the plasmid we have transformed before. | ||
+ | Observation: all transformation except BBa-I15010 | ||
+ | <br /> | ||
+ | |||
+ | <span style="font-family:Verdana; font-weight:700;"> 06/10/2011 </span> | ||
+ | <br /> | ||
+ | 1. Freeze the inoculated culture | ||
+ | <br /> | ||
+ | 2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others | ||
+ | <br /> | ||
+ | |||
+ | <span style="font-family:Verdana; font-weight:700;"> 06/11/2011 </span> | ||
+ | <br /> | ||
+ | 1. Purify plasmid DNA from the culture | ||
+ | <br /> |
Latest revision as of 03:55, 29 September 2011
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Week 1:
06/07/2011
Transformation plasmids into DH5α Competent cells
Plasmids are from iGEM plates:
A. Plate 2 Well 7E (AHL signaling)
B. Plate 3 Well 20F (LovTAP composite)
C. Plate 4 Well 16O (LovTAP Composite)
D. Plate 1 Well 2B (GFP+PEST191)
E. Plate 2 Well 4A (Yeast ADH1 promoter)
06/08/2011
Transformation of E. Coli, Plasmids are from iGEM plates:
Plasmids are from iGEM plates:
A. Plate 2 Well 13J
B. Plate 1 Well 20F
C. Plate 4 Well 16M
D. Plate 1 Well 16K
E. Plate 3 Well 14H
F. Plate 4 Well 6O
06/09/2011
1. Inoculation for all the plasmid we have transformed before.
Observation: all transformation except BBa-I15010
06/10/2011
1. Freeze the inoculated culture
2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others
06/11/2011
1. Purify plasmid DNA from the culture