Team:Caltech/Notebook

From 2011.igem.org

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__NOTOC__
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This is a template page. READ THESE INSTRUCTIONS.
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==Notebook==
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
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[[Team:Caltech/Protocols|Methods and Protocols]]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Caltech|Home]]
 
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!align="center"|[[Team:Caltech/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Caltech Official Team Profile]
 
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!align="center"|[[Team:Caltech/Project|Project]]
 
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!align="center"|[[Team:Caltech/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Caltech/Modeling|Modeling]]
 
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!align="center"|[[Team:Caltech/Notebook|Notebook]]
 
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!align="center"|[[Team:Caltech/Safety|Safety]]
 
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!align="center"|[[Team:Caltech/Attributions|Attributions]]
 
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==Notebook==
 
<p>[[Team:Caltech/Week_1|Week 1]]: 6/15-6/17: Lab orientation</p>
<p>[[Team:Caltech/Week_1|Week 1]]: 6/15-6/17: Lab orientation</p>
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<p>[[Team:Caltech/Week_2|Week 2]]: 6/19-6/25: Lab training; sample collection, enrichment cultures</p>
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<p>[[Team:Caltech/Week_2|Week 2]]: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples</p>
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<p>[[Team:Caltech/Week_3|Week 3]]: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures</p>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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<p>[[Team:Caltech/Week_4|Week 4]]: 7/3-7/10: Sequencing and testing of Gibson assembled vectors</p>
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<p>[[Team:Caltech/Week_5|Week 5]]: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit</p>
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<p>[[Team:Caltech/Week_6|Week 6]]: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit</p>
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<p>[[Team:Caltech/Week_7|Week 7]]: 7/24-7/30: p450 testing, plating of EDC's, more assembly</p>
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<p>[[Team:Caltech/Week_8|Week 8]]: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating</p>
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<p>[[Team:Caltech/Week_9|Week 9]]: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene</p>
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<p>[[Team:Caltech/Week_10|Week 10]]: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene</p>
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<p>[[Team:Caltech/Week_11|Week 11]]: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors</p>
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<p>[[Team:Caltech/Week_12|Week 12]]: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC</p>
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<p>[[Team:Caltech/Week_13|Week 13]]: 9/4-9/10: Quikchange, Biobrick, sequencing</p> 
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<p>[[Team:Caltech/Week_14|Week 14]]: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC</p>
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<p>[[Team:Caltech/Week_15|Week 15]]: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A</P>
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<!-- You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well. -->
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}}

Latest revision as of 20:28, 26 September 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

Notebook

Methods and Protocols

Week 1: 6/15-6/17: Lab orientation

Week 2: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples

Week 3: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures

Week 4: 7/3-7/10: Sequencing and testing of Gibson assembled vectors

Week 5: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit

Week 6: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit

Week 7: 7/24-7/30: p450 testing, plating of EDC's, more assembly

Week 8: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating

Week 9: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene

Week 10: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene

Week 11: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors

Week 12: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC

Week 13: 9/4-9/10: Quikchange, Biobrick, sequencing

Week 14: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC

Week 15: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A


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