Team:Caltech/Week 15

September 18

Quikchange of Wt-F87A plasmid and controls, followed by plating

Results

There are about twenty colonies on the Wt-F87A plates to be sequenced

September 19

Finish desalting purified DDT-dechlorinase of imidazole
Centrifuge concentration of purified DDT-dechlorinase

Results

Estimated concentration of final purified DDT-dechlorinase is .216mg/mL

September 20

Miniprepped and sequenced colonies for quikchange.
12 reactions, 3 primers for each colony:
p450qc-1ABC, p450qc-2ABC, p450qc-3ABC, p450qc-4ABC.
Digest and ligate bisdA, bisdB, & bisdA+bisdB part into correct vector (pSB1C3).