Team:Caltech/Notebook

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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Caltech_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Caltech_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Caltech | Team Example]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Caltech|Home]]
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!align="center"|[[Team:Caltech/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Caltech Official Team Profile]
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!align="center"|[[Team:Caltech/Project|Project]]
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!align="center"|[[Team:Caltech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Caltech/Modeling|Modeling]]
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!align="center"|[[Team:Caltech/Notebook|Notebook]]
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!align="center"|[[Team:Caltech/Safety|Safety]]
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!align="center"|[[Team:Caltech/Attributions|Attributions]]
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==Notebook==
==Notebook==
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[[Team:Caltech/Week_1|Week 1]]
 
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 
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<p>June 21, 2011:<br/>
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[[Team:Caltech/Protocols|Methods and Protocols]]
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9:30 Meeting with Rich <br/>
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10:30-12:00 Overview with Joe and Emzo and lab walkthrough<br/>
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1:00-2:00 Shopping Trip
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(Or is it 1:00-3:00?)<br/>
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2:00-7:15 Lab  
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<p>[[Team:Caltech/Week_1|Week 1]]: 6/15-6/17: Lab orientation</p>
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* LB/Agar Plates
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<p>[[Team:Caltech/Week_2|Week 2]]: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples</p>
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* Glycerol Stock
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<p>[[Team:Caltech/Week_3|Week 3]]: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures</p>
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* Enrichment Media Solution
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<p>[[Team:Caltech/Week_4|Week 4]]: 7/3-7/10: Sequencing and testing of Gibson assembled vectors</p>
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* Transformation of DNA
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<p>[[Team:Caltech/Week_5|Week 5]]: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit</p>
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* Plates
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<p>[[Team:Caltech/Week_6|Week 6]]: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit</p>
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* Competent Cell Culture</p>
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<p>[[Team:Caltech/Week_7|Week 7]]: 7/24-7/30: p450 testing, plating of EDC's, more assembly</p>
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<p>[[Team:Caltech/Week_8|Week 8]]: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating</p>
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<p>[[Team:Caltech/Week_9|Week 9]]: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene</p>
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<p>[[Team:Caltech/Week_10|Week 10]]: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene</p>
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<p>[[Team:Caltech/Week_11|Week 11]]: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors</p>
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<p>[[Team:Caltech/Week_12|Week 12]]: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC</p>
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<p>[[Team:Caltech/Week_13|Week 13]]: 9/4-9/10: Quikchange, Biobrick, sequencing</p> 
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<p>[[Team:Caltech/Week_14|Week 14]]: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC</p>
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<p>[[Team:Caltech/Week_15|Week 15]]: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A</P>
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Latest revision as of 20:28, 26 September 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

Notebook

Methods and Protocols

Week 1: 6/15-6/17: Lab orientation

Week 2: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples

Week 3: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures

Week 4: 7/3-7/10: Sequencing and testing of Gibson assembled vectors

Week 5: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit

Week 6: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit

Week 7: 7/24-7/30: p450 testing, plating of EDC's, more assembly

Week 8: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating

Week 9: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene

Week 10: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene

Week 11: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors

Week 12: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC

Week 13: 9/4-9/10: Quikchange, Biobrick, sequencing

Week 14: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC

Week 15: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A


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