Team:EPF-Lausanne/Our Project/Data

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=== Summary ===
=== Summary ===
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There are three pillars that underlie our transcription factor development pipeline. The first pillar is a selection system which lyses cells containing strong matches between a transcription factor and its promoter and allows for the speedy recovery of the relevant DNA. The second pillar is an in vivo characterization system that uses two different reporter systems as a way to document the binding affinities of the TetR transcription factor and its promoter pTet. The third pillar is an in-vitro characterization system called MITOMI that allows for high-throughput analysis of DNA-protein interaction strengths.  
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[[File:EPFL-Summary_drawing.png|right|thumb|250px]]
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In developing these three pillars, a number of parts were made. We have listed all of them and have highlighted our favorites.  
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Three components constitute our transcription factor development pipeline. The first is a selection system which lyses cells containing strong matches between a mutated transcription factor and its promoter and allows for the speedy recovery of the relevant DNA. The second component is an ''in vivo'' characterization system that uses two different reporter systems as a way to document the binding affinities of the TetR transcription factor and its promoter pTet. The third component is an ''in vitro'' characterization system called MITOMI that allows high-throughput quantitative analysis of DNA-protein interaction strengths.
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In developing these three pillars, a number of parts were made. We have listed all of them here and have highlighted our favorites.
=== Systems ===
=== Systems ===

Latest revision as of 22:05, 28 October 2011