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Team:Glasgow/Results/MCS
From 2011.igem.org
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<h2>Aims</h2> | <h2>Aims</h2> | ||
| - | - To | + | <h6><a href="https://2011.igem.org/Team:Glasgow/Results">Back to Results</a></h6> |
| - | + | ||
| + | - To develop a more efficient method for testing large numbers of biobricks<br/> | ||
<h2>Method</h2> | <h2>Method</h2> | ||
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<br/><Br/> | <br/><Br/> | ||
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | ||
| - | <table border="1"> | + | <table border="1" width="890px"> |
<tr> | <tr> | ||
<td>Forward Primer</td> | <td>Forward Primer</td> | ||
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<tr> | <tr> | ||
<td>Reverse Primer</td> | <td>Reverse Primer</td> | ||
| - | <td>5'- | + | <td> |
| + | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC<br/> | ||
| + | CTCTAGAAGCGGCCGCG-3' | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 03:54, 22 September 2011
Aims
Back to Results
- To develop a more efficient method for testing large numbers of biobricksMethod
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
| Forward Primer | 5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3' |
| Reverse Primer |
5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC CTCTAGAAGCGGCCGCG-3' |
The part is still awaiting synthesis.
You can visit the Multiple Cloning Site Part Page here
