Team:Glasgow/Results/MCS

From 2011.igem.org

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<h2>Aims</h2>
<h2>Aims</h2>
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- To produce an easier method for testing large numbers of biobricks<br/>
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<h6><a href="https://2011.igem.org/Team:Glasgow/Results">Back to Results</a></h6>
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- To reduce the overall number of ligations needed to do this
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- To develop a more efficient method for testing large numbers of biobricks<br/>
<h2>Method</h2>
<h2>Method</h2>
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<center><img src=https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png></center>
<center><img src=https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png></center>
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<br/><Br/>
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We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
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We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/>
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<table border="1" width="890px">
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<u>Forward Primer</u><br/><br/>
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<tr>
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<td>Forward Primer</td>
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                    5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca
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<td>
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                5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3'   
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                    agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt
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</tr>
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<tr>
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                    ttttactagtagcggccgctgca-3'
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<td>Reverse Primer</td>
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<td>
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5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC<br/>
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CTCTAGAAGCGGCCGCG-3'    
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<br/><br/><u>Reverse Primer</u><br/><br/>
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</tr>
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</table>  
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        5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG
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<br/>
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The part is still awaiting synthesis.<br/><br/>
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                    TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT
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                    CTAGAAGCGGCCGCG-3'
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You can visit the Multiple Cloning Site Part Page <a href=https://2011.igem.org/Team:Glasgow/mcs>here</a>

Latest revision as of 03:54, 22 September 2011

Aims

Back to Results
- To develop a more efficient method for testing large numbers of biobricks

Method

The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.



We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:

Forward Primer 5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3'
Reverse Primer 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC
CTCTAGAAGCGGCCGCG-3'

The part is still awaiting synthesis.

You can visit the Multiple Cloning Site Part Page here

Retrieved from "http://2011.igem.org/Team:Glasgow/Results/MCS"