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Team:EPF-Lausanne/Our Project/Attributions
From 2011.igem.org
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iGEM being a competition between students, we worked as independently as possible. Once having been taught all the useful procedures, we did them on our own and analyzed the data before presenting them to the instructors and teaching assistants on our weekly meetings. We also planned the experiments needed to be done each week. | iGEM being a competition between students, we worked as independently as possible. Once having been taught all the useful procedures, we did them on our own and analyzed the data before presenting them to the instructors and teaching assistants on our weekly meetings. We also planned the experiments needed to be done each week. | ||
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| + | == T7 Lysis == | ||
| + | {| | ||
| + | ! Which task | ||
| + | ! Student(s) | ||
| + | ! Assistant(s) | ||
| + | |- | ||
| + | | Primer design | ||
| + | | Alessandro | ||
| + | | Henrike | ||
| + | |- | ||
| + | | PCR and Assembly | ||
| + | | Alessandro, Vincent | ||
| + | | Henrike | ||
| + | |- | ||
| + | | Characterization | ||
| + | | Vincent | ||
| + | | Henrike | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | |||
Latest revision as of 17:20, 21 September 2011
Attributions & Contributions
iGEM being a competition between students, we worked as independently as possible. Once having been taught all the useful procedures, we did them on our own and analyzed the data before presenting them to the instructors and teaching assistants on our weekly meetings. We also planned the experiments needed to be done each week.
Being a small group, each student specialized on one part of the project, such as using the MITOMI or creating the T7 promoter variants. This allowed us to spend less time on learning the procedures; especially true for the microfluidics setups. The different parts of the project are detailed below, with the name of the student that worked on each task. The teaching assistants were assigned to the different tasks based on their personal competence; this does not mean that they completed the task, but rather that they taught us how to do it and answered our questions relative to it.
Contents |
TetR mutants
Site-directed mutagenesis
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Clara | Alina |
| Cloning | Douglas, Vincent | Alina |
| In vivo characterization | Nadine | |
| Biobrick | Lilia |
MITOMI
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| In vitro characterization | Lilia | Alina, Matt |
T7 promoter variants
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Alessandro | Henrike |
| Cloning | Alessandro, Vincent | |
| In vivo characterization | Alessandro, Vincent | |
| Biobrick | Nadine |
T7 Lysis
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Alessandro | Henrike |
| PCR and Assembly | Alessandro, Vincent | Henrike |
| Characterization | Vincent | Henrike |
Reporter plasmids
J61002 Ptet-RFP
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Henrike | |
| Cloning | Vincent | Irina |
| In vivo characterization | Nadine, Alessandro |
J61002 Plac-RFP
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Nadine | |
| Cloning | Nadine, Alessandro | |
| In vivo characterization | Nadine | |
| Biobrick of Plac | Nadine | Sequence from Henrike |
J61002 Plac-lysis
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Nadine | |
| Cloning | Nadine, Alessandro | |
| In vivo characterization |
pSB3K1 Pconst-TetR
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Alessandro | Henrike |
| Cloning | Nadine, Alessandro | Irina, Henrike |
| In vivo characterization | Nadine | Henrike |
pSB3K1 Pconst-TetR Ptet-LacI
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Primer design | Nadine | |
| Cloning | Nadine | |
| In vivo characterization | Nadine |
Microfluidics chips
Worm chip
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Chip design | Clara, Douglas | Sebastian |
| Wafer fabrication | Clara | |
| Chip fabrication | Clara | Henrike |
| Building the game setup | Douglas | |
| Programming the web interface | Douglas |
MITOMI chip
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Chip design | Existing design | |
| Chip fabrication | Lilia, Clara | |
| Experiments | Lilia | Alina, Matt |
Wiki
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Layout | Douglas | |
| Logo design | Solange | |
| Team banner, logo colouring | Douglas | |
| Safety Page | Vincent | |
| Specialized pages | According to our work | |
| General pages | Various team members | |
| Graphing and Reporting | Douglas, Vincent | Irina |
| Attributions page | Nadine |
Misc
| Which task | Student(s) | Assistant(s) |
|---|---|---|
| Agar plates | Lilia | |
| Antibiotics aliquots | Lilia | |
| Lab entertainment | Minnie mouse, the Hawaiian girl, the Pirate | |
| Stress level managers | Bart, Sebastian | |
| Cakes | Matt, the LBNC lab |
