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Team:Glasgow/2011.igem.org/Team:Glasgow/Ranaspumin2 Sequence
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| - | Rsn2(His-tagged) | + | == Rsn2(His-tagged) == |
| + | Nucleotide Sequence: | ||
gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC | gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC | ||
| Line 9: | Line 10: | ||
| - | TAATAA: introduced double stop codon | + | TAATAA: introduced double stop codon. |
Lowercase: biobrick ends on primers. | Lowercase: biobrick ends on primers. | ||
| + | |||
No illegal restriction sites. | No illegal restriction sites. | ||
| + | == Rsn2(non-His-tagged) == | ||
| - | + | Nucleotide Sequence: | |
| - | + | ||
gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT | gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT | ||
| Line 25: | Line 27: | ||
| - | + | TAATAA: introduced double stop codon. | |
| + | |||
Lowercase: biobrick ends on primers. | Lowercase: biobrick ends on primers. | ||
| + | |||
No illegal restriction sites. | No illegal restriction sites. | ||
| + | == Protein Sequence == | ||
| - | + | 117 Amino Acids: | |
MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS | MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS | ||
VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY | VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY | ||
Latest revision as of 22:21, 20 September 2011
Rsn2(His-tagged)
Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACAATCTGTTTGGTAAAGAACTC CTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGACATCAGAAGATAAAAGGTTTT ACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCAAAGATGGTCGGACGCAAATG TAGGAAGGACGATGATGATGATGATGG(ttac)TAATAAtactagtagcggccgctgcagacacac
TAATAA: introduced double stop codon.
Lowercase: biobrick ends on primers.
No illegal restriction sites.
Rsn2(non-His-tagged)
Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT CACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACA ATCTGTTTGGTAAAGAACTCCTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGAC ATCAGAAGATAAAAGGTTTTACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCA AAGATGGTCGGACGCAAATGTAGGAAGGACGATGATGATGATGATGGTAATAAtactagtagcggccgctgcagacacac
TAATAA: introduced double stop codon.
Lowercase: biobrick ends on primers.
No illegal restriction sites.
Protein Sequence
117 Amino Acids:
MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY
