Team:Paris Liliane Bettencourt/Notebook/2011/09/20/

(Difference between revisions)

Latest revision as of 21:01, 20 September 2011

Team IGEM Paris 2011

Control digestion of pHM3 in NcoI

Control of the size of the insert

S75 *5; S82 *5; 104 *5

ComS *3

We characterized T7 autoloop by microscopy. Leak,

T7 autoloop RFP+ Control / E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

TEXT

T7 autoloop full no terminator / E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

T7 autoloop full with terminator / E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

We

TEXT

@@ Line 8: / Line 8: @@
 [[File:CP2009.jpg|thumb|center]]
+Control of the size of the insert
+[[File:CP2009_XP.jpg|thumb|center|S75 *5; S82 *5; 104 *5]]
+[[File:CP2009_XP_ComS.jpg|thumb|center|ComS *3]]
 == Baptiste, Hovannes & Ouriel ==
@@ Line 38: / Line 39: @@
 {| border="1" class="wikitable" style="text-align: center;"
-|+T7 autoloop full no double terminator / E-coli at 37°C
+|+T7 autoloop full no terminator / E-coli at 37°C
 |-
 |[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
@@ Line 51: / Line 52: @@
 {| border="1" class="wikitable" style="text-align: center;"
-|+T7 autoloop full with double terminato / E-coli at 37°C
+|+T7 autoloop full with  terminator / E-coli at 37°C
 |-
 |[[File:T7_loop_1.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]