Team:Paris Liliane Bettencourt/Notebook/2011/09/20/

From 2011.igem.org

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[[File:CP2009.jpg|thumb|center]]
[[File:CP2009.jpg|thumb|center]]
 +
Control of the size of the insert
-
 
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[[File:CP2009_XP.jpg|thumb|center|S75 *5; S82 *5; 104 *5]]
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[[File:CP2009_XP_ComS.jpg|thumb|center|ComS *3]]
== Baptiste, Hovannes & Ouriel ==
== Baptiste, Hovannes & Ouriel ==
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{| border="1" class="wikitable" style="text-align: center;"
{| border="1" class="wikitable" style="text-align: center;"
-
|+T7 autoloop RFP+ Control in E-coli at 37°C
+
|+T7 autoloop RFP+ Control / E-coli at 37°C
|-
|-
-
|[[File:0915_3610_stat2_w1TRANS_s1_t1.jpg|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=0min position 1 (trans image)]]
+
|[[File:T7 autoloop ctrl-7.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0915_3610_stat2_w1TRANS_s1_t1.jpg|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=0min position 1 (trans image)]]
+
|[[File:T7 autoloop ctrl-8.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
-
|[[File:0915_3610_stat2_w2GFP_s1_t1.png|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=0min position 1 (gfp image)]]
+
|[[File:T7 autoloop ctrl-9.jpg|290px|thumb|center|E-Coli  at 37°C (rfp image)]]
|-
|-
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|[[File:0915_3610_stat2_refocused_w1TRANS_s1_t11.png|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=120min position 1 (trans image)]]
+
|[[File:T7 autoloop ctrl-10.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0915_3610_stat2_w1TRANS_s1_t1.jpg|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=0min position 1 (trans image)]]
+
|[[File:T7 autoloop ctrl-11.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
-
|[[File:0915_3610_stat2_refocused_2GFP_s1_t11.png|300px|thumb|center|3610 gfp- and 3610 gfp+ strains stationary phase at 37°C t=120min position 1 (gfp image)]]
+
|[[File:T7 autoloop ctrl-12.jpg|290px|thumb|center|-Coli  at 37°C (rfp image)]]
|}
|}
-
 
-
 
-
 
-
 
-
===YFP-tetR===
 
-
 
-
We
 
-
 
-
 
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{| border="1" class="wikitable" style="text-align: center;"
{| border="1" class="wikitable" style="text-align: center;"
-
|+3610 gfp-/3610 gfp+ in exponantial phase at 37°C
+
|+T7 autoloop full no terminator / E-coli at 37°C
|-
|-
-
|[[File:0914_3610_classic_w1TRANS_s5_t1.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=0min position 5 (trans image)]]
+
|[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0914_3610_classic_w2GFP_s5_t1.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=0min position 5 (gfp image)]]
+
|[[File:T7 autoloop ctrl-26.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
 +
|[[File:T7 autoloop ctrl-27.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
|-
|-
-
|[[File:0914_3610_classic_w1TRANS_s5_t9.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=45min position 5 (trans image)]]
+
|[[File:T7 autoloop ctrl-31.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0914_3610_classic_w2GFP_s5_t9.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=45min position 5 (gfp image)]]
+
|[[File:T7 autoloop ctrl-32.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
 +
|[[File:T7 autoloop ctrl-33.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
 +
|}
 +
 
 +
 
 +
{| border="1" class="wikitable" style="text-align: center;"
 +
|+T7 autoloop full with  terminator / E-coli at 37°C
|-
|-
-
|[[File:0914_3610_classic_w1TRANS_s5_t12.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=60min position 5 (trans image)]]
+
|[[File:T7_loop_1.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0914_3610_classic_w2GFP_s5_t12.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ at 37°C t=60min position 5 (gfp image)]]
+
|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
 +
|[[File:T7 loop 1 rfp.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
|-
|-
-
|[[File:0914_3610_classic_w1TRANS_s5_t23.png|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=180min position 5 (trans image)]]
+
|[[File:T7 loop 2.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
-
|[[File:0914_3610_classic_w2GFP_s5_t23.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=180min position 5 (gfp image)]]
+
|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
 +
|[[File:T7 loop 2 rfp.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
|}
|}
 +
 +
===YFP-tetR===
 +
 +
We
 +
TEXT
TEXT

Latest revision as of 21:01, 20 September 2011

Team IGEM Paris 2011


Contents

Cyrille

Control digestion of pHM3 in NcoI

CP2009.jpg

Control of the size of the insert

S75 *5; S82 *5; 104 *5
ComS *3

Baptiste, Hovannes & Ouriel

Characterisation of T7 autoloop, RFP - GFP

We characterized T7 autoloop by microscopy. Leak,


T7 autoloop RFP+ Control / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


TEXT


T7 autoloop full no terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


T7 autoloop full with terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)

YFP-tetR

We


TEXT

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