Team:Valencia/Notebook/Week8

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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia"><b>Home</b></a>
 
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Team"><b>The team</b></a>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Acknowledgements">Acknowledgements & Attributions</a></li>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Gallery">Gallery</a></li>
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Project"><b>The project</b></a>
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<div class=col_center_top><b>WEEK 8</b></div><!-- fin clase col_left_top-->
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Achievements">Achievements</a></li>
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<b>Engineering:</b> We found a better solution for the bacteriocins separator module. It works, but we still have in mind to find a more efficient solution.  
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Hardware">Hardware</a></li>
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We built the magnetic mixers with a computer fan and the magnets from the hard disk. We have realized how under pressure you can find different innovative solutions.
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project1">Colicin production</a></li>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project2">pH-stat</a></li>
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<b>pH-stat:</b> The cultures of the previous week were colourless, and we also found out  with  a microscope that they had no living cells. So, two new cultures with the same characteristics were set up. The day after ,we could observe that there was no cell growth, but a concentration decrease. The causes were not well known . It is suspected that the inactivity of the culture  might  be due to  these facts:  
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Modeling">Modeling</a></li>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Notebook/Calender">Notebook</a></li>
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<li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Protocols">Protocols</a></li>
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<p> 1. The room temperature  had not  been  constant, varying  from 30 °  at room temperature  to  22 º C  at refrigeration temperature.</p>  
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<p> 2. The concentration of fertilizer was  not enough to motivate the proliferation. </p>
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Parts"><b>BioBricks</b></a>
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<p> 3.The excess of light caused by the two  tubes plus the reflector / spotlight  caused photoinhibition (Portilla, A. et. Al., 2009). </p>  
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Safety"><b>Human Practices</b></a>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safetyquestions">Biosafety and Biosecurity</a></li>
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Experts">Asking the experts</a></li>
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<div class=col_center_top><b>WEEK 8</b></div><!-- fin clase col_left_top-->
 
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<div class=col_center_center>We find a better solution for the bacteriocins separator module. It works, but we still have in mind to find a better solution.
 
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We build the magnetic mixers with a computer fan and the magnets from the hard disc. We realize how your mind can find different solutions when you have limited means.
 
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<b>Hardware</b>: Reading papers. We need to know as much as possible about bacteriocins.
 
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<b>pH-stat</b>  
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<b>Colicin production:</b> We finally purchase two bacteriocin-producing strains at Health Protection Agency.<br>
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<b>Colicin production</b>
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Latest revision as of 03:55, 22 September 2011



WEEK 8
Engineering: We found a better solution for the bacteriocins separator module. It works, but we still have in mind to find a more efficient solution. We built the magnetic mixers with a computer fan and the magnets from the hard disk. We have realized how under pressure you can find different innovative solutions.

pH-stat: The cultures of the previous week were colourless, and we also found out with a microscope that they had no living cells. So, two new cultures with the same characteristics were set up. The day after ,we could observe that there was no cell growth, but a concentration decrease. The causes were not well known . It is suspected that the inactivity of the culture might be due to these facts:

1. The room temperature had not been constant, varying from 30 ° at room temperature to 22 º C at refrigeration temperature.

2. The concentration of fertilizer was not enough to motivate the proliferation.

3.The excess of light caused by the two tubes plus the reflector / spotlight caused photoinhibition (Portilla, A. et. Al., 2009).



Colicin production: We finally purchase two bacteriocin-producing strains at Health Protection Agency.
Notebook Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Project

Retrieved from "http://2011.igem.org/Team:Valencia/Notebook/Week8"