"
Team:Valencia/Notebook/Week6
From 2011.igem.org
(Difference between revisions)
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Template:Valencia-0-3}} | ||
<html> | <html> | ||
| - | |||
<body> | <body> | ||
| - | |||
<style type="text/css"> | <style type="text/css"> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
.center { | .center { | ||
| Line 30: | Line 23: | ||
.col_center_top{ | .col_center_top{ | ||
width:908px; | width:908px; | ||
| - | background-color:# | + | background-color:#0087ba; |
height:20px; | height:20px; | ||
font-family:Verdana, Arial, Helvetica, sans-serif; | font-family:Verdana, Arial, Helvetica, sans-serif; | ||
| Line 60: | Line 53: | ||
padding:20px; | padding:20px; | ||
} | } | ||
| + | |||
| Line 164: | Line 158: | ||
if (window.attachEvent) window.attachEvent("onload", sfHover); | if (window.attachEvent) window.attachEvent("onload", sfHover); | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
body { | body { | ||
font: 10px normal Verdana, Arial, Helvetica, sans-serif; | font: 10px normal Verdana, Arial, Helvetica, sans-serif; | ||
| Line 313: | Line 255: | ||
}); | }); | ||
</script> | </script> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<div class=center> | <div class=center> | ||
| Line 369: | Line 262: | ||
<div class=col_center_top><b>WEEK 6</b></div><!-- fin clase col_left_top--> | <div class=col_center_top><b>WEEK 6</b></div><!-- fin clase col_left_top--> | ||
<div class=col_center_center> | <div class=col_center_center> | ||
| - | <b> | + | <b>Engineering</b>:Finally we decide to buy a functional motor and try to assemble it with the pump. |
The first bacteriocins separator module is built with a small container (a hair wax box) and a couple of toric joints. The cost is around 5 euros. Take a look to the pictures, is awesome, isn’t it? | The first bacteriocins separator module is built with a small container (a hair wax box) and a couple of toric joints. The cost is around 5 euros. Take a look to the pictures, is awesome, isn’t it? | ||
| - | We | + | We found a dialysis membrane that could work properly to homogenize the pH. |
<br><br> | <br><br> | ||
| - | <b>pH-stat</b> | + | <b>pH-stat:</b> We began the first simultaneous experiment with the established cultures: |
| + | |||
| + | <p> 1. Bottle 1 is placed under continuous light for 24 hours to observe the evolution of the pH.</p> | ||
| + | |||
| + | <p> 2. Bottle 2 is left under the same conditions and a 12h/12h photoperiod.</p> | ||
| + | |||
| + | |||
| + | <p>Initially bottle 1 was 7.63 and bottle 2 was 7.59 but after two days, contrary to expectations, the pH had dropped to 6.56 and 6.23 respectively. So, besides the decrease of pH, the light cycle disorder caused severe damage in the culture, reducing drastically the concentration of <i>Synechocystis</i>. We therefore concluded that the first experiment was a failure.</p> | ||
| + | |||
<br><br> | <br><br> | ||
| - | <b>Colicin production</b> | + | <b>Colicin production:</b> We have sent some e-mails, expecting some experienced researchers to help us with the use of bacteriocins in our experiments. Now to wait for answers!<br> |
</div><!-- fin clase col_left_top--> | </div><!-- fin clase col_left_top--> | ||
| Line 385: | Line 286: | ||
<IMG SRC="https://static.igem.org/mediawiki/2011/0/0e/Valencia_Notebookplastifik.gif" | <IMG SRC="https://static.igem.org/mediawiki/2011/0/0e/Valencia_Notebookplastifik.gif" | ||
| - | WIDTH= | + | WIDTH=606 HEIGHT=526 BORDER=0 ALT="Notebook" USEMAP="#Valencia_Notebook"> |
<MAP NAME="Valencia_notebook"> | <MAP NAME="Valencia_notebook"> | ||
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="88,90,70" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week1" ALT="Week 1"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="232,69,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week2" ALT="Week 2"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="327,125,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week3" ALT="Week 3"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="397,67,30" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week4" ALT="Week 4"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="483,108,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week5" ALT="Week 5"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="547,194,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week6" ALT="Week 6"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="468,245,30" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week7" ALT="Week 7"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="353,305,70" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week8" ALT="Week 8"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="231,231,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week9" ALT="Week 9"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="164,288,30" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week10" ALT="Week 10"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="73,314,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week11" ALT="Week 11"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="106,420,50" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week12" ALT="Week 12"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="200,430,30" HREF="https://2011.igem.org/Team:Valencia/Notebook/Week13" ALT="Week 13"> |
| - | <AREA SHAPE=CIRCLE COORDS=" | + | <AREA SHAPE=CIRCLE COORDS="295,468,50" HREF="https://2011.igem.org/Team:Valencia/Project" ALT="Project"> |
| - | </MAP> | + | |
| + | </MAP> | ||
| + | |||
Latest revision as of 03:52, 22 September 2011
WEEK 6
Engineering:Finally we decide to buy a functional motor and try to assemble it with the pump.
The first bacteriocins separator module is built with a small container (a hair wax box) and a couple of toric joints. The cost is around 5 euros. Take a look to the pictures, is awesome, isn’t it?
We found a dialysis membrane that could work properly to homogenize the pH.
pH-stat: We began the first simultaneous experiment with the established cultures:
Colicin production: We have sent some e-mails, expecting some experienced researchers to help us with the use of bacteriocins in our experiments. Now to wait for answers!
pH-stat: We began the first simultaneous experiment with the established cultures:
1. Bottle 1 is placed under continuous light for 24 hours to observe the evolution of the pH.
2. Bottle 2 is left under the same conditions and a 12h/12h photoperiod.
Initially bottle 1 was 7.63 and bottle 2 was 7.59 but after two days, contrary to expectations, the pH had dropped to 6.56 and 6.23 respectively. So, besides the decrease of pH, the light cycle disorder caused severe damage in the culture, reducing drastically the concentration of Synechocystis. We therefore concluded that the first experiment was a failure.
Colicin production: We have sent some e-mails, expecting some experienced researchers to help us with the use of bacteriocins in our experiments. Now to wait for answers!
