Team:Arizona State

From 2011.igem.org

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We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition. Our Wiki will be updated as our project progresses, so check back often.
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We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.
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<center>[[Image:ASU_tips.png|400px]]</center>
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== Abstract ==
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<p>Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.</p>
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'''What
 is
 iGEM?
'''
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''[[Team:Arizona State/Project/Introduction|more]]''
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----
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The International 
Genetically 
Engineered 
Machine
 (iGEM) 
competition
 is 
the 
premiere
 undergraduate
 synthetic 
biology
competition, 
encouraging 
students 
around 
the 
world 
to 
develop
 novel 
applications
 for
 genetic 
engineering.
 The
  competition 
has 
grown 
from 
5 
universities 
in 
2004
 to 
over 
160 
universities 
in 
2011 
worldwide. 
This 
year 
iGEM 
will 
be
holding 
a 
regional
 competition
 for 
the 
Americas 
at 
the 
Institute 
for 
Biological 
Engineering 
in 
Indianapolis, 
where
 a
 portion 
of
 contestant 
universities
 will 
move 
on 
to 
the 
World
 Championship.
 The
 World 
Championship 
will 
be
 held 
at 
the
 Massachusetts 
Institute 
of 
Technology
 in 
November
2011.
 Projects
 are 
judged 
by 
their
 innovation 
and 
application 
of 
gene
 networks 
to 
form 
products 
with 
novel
 functions.
 Examples 
of
 past 
projects 
include
 a 
H.
 pylori
 vaccination
 candidate,
 bacteria
 that 
solve 
sudoku 
puzzles,
 heavy
 metal 
detection 
using 
E.
coli, 
and 
a 
waterborne 
parasite
 detection 
system. 
As
 ASU's 
inaugural 
team
 we 
plan 
to 
focus 
on 
NDM‐1 
acquired 
antibiotic 
resistance 
and 
the 
CRISPR‐Cas 
pathway.

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'''Global
Challenge
'''
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-
----
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-
The
WHO
and
CDC
consider
antibiotic
resistance
to
be
one
of
the
most
pressing
global
health

+
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threats,
compromising
the
effectiveness
of
our
most
important
tool
in
fighting
bacterial
infection.

+
-
NDM‐1
allows
bacteria
to
be
resistant
to
a
broad
range
of
commonly
used
antibiotics
and
has

+
-
spread
rapidly
from
India
and
Pakistan
throughout
the
world.
Alternative
solutions
must
be

+
-
promptly
and
intelligently
employed
to
counter
this
threat.

+
-
'''Bacterial
Immunity
'''
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-
----
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-
CRISPR
functions
as
a
bacterial
immune
system
by
incorporating
and
targeting
foreign
genetic

+
-
elements.
The
modularity
of
this
system
presents
the
opportunity
for
unique
applications.
Our

+
-
innovative
solution
uses
CRISPR
to
silence
the
NDM‐1
gene,
consequently
developing
a
new

+
-
method
that
can
be
utilized
for
many
novel
gene
manipulations
in
bacteria.
This
project
combats

+
-
an
important
global
issue
with
cutting‐edge
science.
As
Arizona
State
University’s
first
iGEM
team,

+
-
we
have
chosen
an
ambitious
project
that
has
great
potential
to
advance
the
field
of
synthetic

+
-
biology,
bringing
an
international
spotlight
to
ASU
and
the
Phoenix
community.


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[[Image:Arizona_State_proposal_diagram0.png]]
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<html><a href="mailto:asu.igem@gmail.com">Contact us</a></html>
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== What is CRISPR? ==
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<p>'''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.</p>
<center>
<center>
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[[Image:ASU Crispr basic.png|600px|Basic mechanism]]<br>
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<html>
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A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. '''[[Team:Arizona State/Project/CRISPR|More information]]'''.
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[[Image:Arizona_State_logo_igem.png|center]]
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}}

Latest revision as of 03:17, 29 September 2011



Home iGEM 2011 Home Project
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We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.
ASU tips.png

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.

more

What is CRISPR?

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.

Basic mechanism
A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. More information.


Contact Us

Arizona State University

ECG 334, PO BOX 9709

Tempe, Arizona 85287

Email

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