Team:Imperial College London/Sequence

From 2011.igem.org

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Line 3: Line 3:
<body>
<body>
<h1>Sequencing Results</h1>
<h1>Sequencing Results</h1>
-
<p><b>Vector 7 forward sequencing result</b></p>
+
<h2>Vector 7 forward sequencing result</h2>
<p> BBa_K316001 (7)_premix -- 19..973 of sequence </p>
<p> BBa_K316001 (7)_premix -- 19..973 of sequence </p>
Line 24: Line 24:
CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA</p>
CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA</p>
-
<p><b>Vector 8 forward sequencing result</b></p>
+
<h2>Vector 8 forward sequencing result</h2>
<p>BBa_K316005 (8)_premix -- 17..635 of sequence</p>
<p>BBa_K316005 (8)_premix -- 17..635 of sequence</p>
Line 39: Line 39:
CTGGAAAGTGGGGTTTGAA</p>
CTGGAAAGTGGGGTTTGAA</p>
-
<p><b>Vector 6 forward sequencing result</b></p>
+
<h2>Vector 6 forward sequencing result</h2>
<p>BBa_K398500 (6)_premix -- 17..960 of sequence</p>
<p>BBa_K398500 (6)_premix -- 17..960 of sequence</p>
Line 59: Line 59:
ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT</p>
ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT</p>
-
<p><b>?</b></p>
+
<h2>?</h2>
<p>PSVi34-1_premix -- 19..951 of sequence</p>
<p>PSVi34-1_premix -- 19..951 of sequence</p>
Line 79: Line 79:
AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG</p>
AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG</p>
-
<p><b>?</b></p>
+
<h2>?</h2>
<p>PSVi34-2_premix -- 21..949 of sequence</p>
<p>PSVi34-2_premix -- 21..949 of sequence</p>
Line 99: Line 99:
GCTAAGATCCGCTCTAACCGAAAAGGAAG</p>
GCTAAGATCCGCTCTAACCGAAAAGGAAG</p>
-
<p><b>Sequence results for possible auxin producing colony</b></p>
+
<h2>Sequence results for J23113 ligation attempt into RFP. Failed.</h2>
<p> 52.E_premix -- 14..957 of sequence</p>
<p> 52.E_premix -- 14..957 of sequence</p>
Line 118: Line 118:
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT</p>
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT</p>
 +
 +
<h2>Sequence results for possible auxin producing colony</h2>
<p>53_AUX1 -- 13..914 of sequence</p>
<p>53_AUX1 -- 13..914 of sequence</p>
Line 168: Line 170:
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA</p>
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA</p>
-
 
-
 
-
<p><b>Sequence results for possible auxin producing colony</b></p>
 
<p>53_premix -- 17..119 of sequence</p>
<p>53_premix -- 17..119 of sequence</p>
Line 193: Line 192:
-
<p><b>Sequence results for possible auxin producing colony</b></p>
+
<h2>Sequence results for possible auxin producing colony</h2>
<p>55_AUX1 -- 18..841 of sequence</p>
<p>55_AUX1 -- 18..841 of sequence</p>
Line 278: Line 277:
ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT</p>
ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT</p>
-
<p><b>Sequence results for possible auxin producing colony</b></p>
+
<h2>Sequence results for our Auxin Xpress module.</h2>
<p>56_AUX1 -- 21..841 of sequence</p>
<p>56_AUX1 -- 21..841 of sequence</p>
Line 295: Line 294:
CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA
CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA
CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p>
CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p>
-
 
-
<p><b>Sequence results for our auxin producing construct</b></p>
 
<p>56_AUX2 -- 16..912 of sequence</p>
<p>56_AUX2 -- 16..912 of sequence</p>
Line 367: Line 364:
TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG
TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG
CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC</p>
CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC</p>
 +
 +
<h2>Sequence results to determine SpeI site problem in sfGFP</h2>
<p>17R_premix -- 35..933 of sequence</p>
<p>17R_premix -- 35..933 of sequence</p>
Line 385: Line 384:
GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA</p>
GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA</p>
-
<p><b>Sequence results for possible PA2652 producing colony</b></p>
+
<h2>Sequence results for possible PA2652 producing colony</h2>
<p>57.20F_premix -- 14..986 of sequence</p>
<p>57.20F_premix -- 14..986 of sequence</p>
Line 425: Line 424:
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG</p>
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG</p>
-
<p><b>Sequence results for possible PA2652 producing colony</b></p>
+
<h2>Sequence results for possible PA2652 producing colony</h2>
<p>57.3F_premix -- 15..985 of sequence</p>
<p>57.3F_premix -- 15..985 of sequence</p>
Line 465: Line 464:
TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT</p>
TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT</p>
-
<p><b>Sequence results for possible PA2652 producing colony</b></p>
+
<h2>Sequence results for possible PA2652 producing colony</h2>
<p>57.7F_premix -- 14..987 of sequence</p>
<p>57.7F_premix -- 14..987 of sequence</p>
Line 505: Line 504:
CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC</p>
CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC</p>
-
<p><b>Sequence results for our PA2652 producing construct</b></p>
+
<h2>Sequence results for our PA2652 producing construct. Correct.</h2>
<p>57.8F_premix -- 15..979 of sequence</p>
<p>57.8F_premix -- 15..979 of sequence</p>
Line 545: Line 544:
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p>
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p>
 +
<h2>Dendra2 forward sequencing results after ligation with Ptet and RBS</h2>
 +
<p> Dendra_forward_premix -- 15..179 of sequence</p>
 +
<p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA
 +
TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA</p>
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
<p> 21+60a_frwrd_premix -- 15..921 of sequence</p>
 +
<p>ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA
 +
AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA
 +
TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC
 +
AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA
 +
ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT
 +
ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT
 +
TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG
 +
GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC
 +
CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC
 +
TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA
 +
CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA
 +
GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG
 +
CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC
 +
ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC
 +
TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG
 +
CTGCAGG</p>
 +
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
 +
<p> 21+60b_frwrd_premix -- 18..928 of sequence</p>
 +
<p>TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA
 +
TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA
 +
GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC
 +
GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC
 +
AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC
 +
AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA
 +
GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG
 +
GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC
 +
CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT
 +
ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC
 +
GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG
 +
CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC
 +
ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG
 +
ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT
 +
GCAGGCTTCCT</p>
 +
 +
<h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2>
 +
 +
<p> 21+60c_frwrd_premix -- 18..926 of sequence</p>
 +
<p>AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA
 +
TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA
 +
GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC
 +
GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC
 +
AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC
 +
AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA
 +
GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG
 +
GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC
 +
CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT
 +
ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC
 +
GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG
 +
CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC
 +
ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT
 +
AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC
 +
AGGCTTCCT</p>
 +
 +
<h2>Results of corrected SpeI site of sfGFP</h2>
 +
 +
<p> sfGFP_reverse_premix -- 20..288 of sequence</p>
 +
<p>ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG
 +
CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC
 +
AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG
 +
GGTGCTCAGATAGTGATTATGCCGGAGCC</p>
-
<p><b>Sequence of Anti-holin colony 2</b></p>
+
<h2>Sequence of Anti-holin colony 2</h2>
<p> 70.2 F_premix -- 25..943 of sequence</p>
<p> 70.2 F_premix -- 25..943 of sequence</p>
<p>AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
<p>AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
Line 587: Line 658:
GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT</p>
GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT</p>
-
<p><b>Sequence of Anti-holin colony 2</b></p>
+
<h2>Sequence of Anti-holin colony 2</h2>
<p> 70.9 F_premix -- 18..1008 of sequence</p>
<p> 70.9 F_premix -- 18..1008 of sequence</p>
Line 627: Line 698:
TTCATCGCTCTGGAGTGA</p>
TTCATCGCTCTGGAGTGA</p>
 +
<h2>Sequence of Dendra2 construct. Verified.</h2>
 +
 +
<p> 71.2F_premix -- 14..961 of sequence
 +
ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA
 +
GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG
 +
TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA
 +
AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC
 +
TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA
 +
AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG
 +
AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG
 +
AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC
 +
CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG
 +
ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC
 +
TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT
 +
TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT
 +
ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT
 +
AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT
 +
AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC
 +
GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT</p>
 +
 +
<p> 71.2R_premix -- 16..995 of sequence
 +
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT
 +
CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA
 +
CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC
 +
AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC
 +
CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA
 +
CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC
 +
CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC
 +
GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT
 +
ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG
 +
GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC
 +
CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT
 +
TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA
 +
TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT
 +
TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT
 +
CGGGCACGTAAGAGGTTCCA</p>
 +
 +
<h2>Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.</h2>
 +
 +
<p> 72F_premix -- 15..135 of sequence
 +
TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG
 +
GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC
 +
C</p>
 +
 +
<p>72R_premix -- 20..851 of sequence
 +
ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT
 +
TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT
 +
CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA
 +
CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC
 +
AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC
 +
CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA
 +
CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC
 +
CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC
 +
GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT
 +
ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG
 +
GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC
 +
CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT
 +
TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT</p>
 +
 +
<h2>J23100-sfGFP composite. Sequence verified.</h2>
 +
 +
 +
<p> 73F_premix -- 22..1003 of sequence
 +
AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG
 +
ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG
 +
TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG
 +
TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT
 +
AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC
 +
TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC
 +
GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG
 +
ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA
 +
ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT
 +
ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC
 +
CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT
 +
CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA
 +
ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG
 +
AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG
 +
GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG
 +
TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT
 +
ACTTCGCGTTATGCAGGCTTCC</p>
 +
<p> 73R_premix -- 19..338 of sequence
 +
GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG
 +
TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC
 +
GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA
 +
CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT
 +
GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT
 +
GCTGGTAATGGTCGGGTTAC</p>
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Latest revision as of 09:52, 20 September 2011



Sequencing Results

Vector 7 forward sequencing result

BBa_K316001 (7)_premix -- 19..973 of sequence

TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCA CCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGGTAACAGGATTAG CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA

Vector 8 forward sequencing result

BBa_K316005 (8)_premix -- 17..635 of sequence

TAGAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTTACTAGAGCTCATGAACTATGAAGAGGTGACGTCATGAACAAAGGTGTAA TGCGACCGGGCCATGTGCAGCTGCGTGTACTGGACATGAGCAAGGCCCTGGAACACTACG TCGAGTTGCTGGGCCTGATCGAGATGGACCGTGACGACCAGGGCCGTGTCTATCTGAAGG CTTGGACCGAAGTGGATAAGTTTTCCCTGGTGCTACGCGAGGCTGACGAGCCGGGCATGG ATTTTATGGGTTTCAAGGTTGTGGATGAGGATGCTCTCCGGCAACTGGAGCGGGATCTGA TGGCATATGGCTGTGCCGTTGAGCAGCTACCCGCAGGTGAACTGAACAGTTGTGGCCGGC GCGTGCGCTTCCAGGCCCCCTCCGGGCATCACTTCGAGTTGTATGCAGACAAGGAATATA CTGGAAAGTGGGGTTTGAA

Vector 6 forward sequencing result

BBa_K398500 (6)_premix -- 17..960 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCTTCTAGAGAAAGAGGGGACAAACTAGATGCGTAAAGGAGAAGAACTTTTC ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC ACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT CAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATG CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG ATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT

?

PSVi34-1_premix -- 19..951 of sequence

CATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCT CAGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAA TTACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTAT TCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTT CTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTT GTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTG TTCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCA TGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGA CCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTA TTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTC ACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTA GACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAA TTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTAT CCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTG GTATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCT AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG

?

PSVi34-2_premix -- 21..949 of sequence

ATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCTC AGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAAT TACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTATT CACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTC TGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTG TACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGT TCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCAT GCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGAC CAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTAT TGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCA CAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAAT TGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATC CAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGG TATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCTA GCTAAGATCCGCTCTAACCGAAAAGGAAG

Sequence results for J23113 ligation attempt into RFP. Failed.

52.E_premix -- 14..957 of sequence

ATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC TCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAAC GGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACC GCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCG CAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTG AAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGT GTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAA CTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGG GAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATG CGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCT AAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCC CACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACC GGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAA TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT

Sequence results for possible auxin producing colony

53_AUX1 -- 13..914 of sequence

GCATCTGATTTTCCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATG AGCACCAGCATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACG TGCCTGAACGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTG CCCCGCGCCCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAG ATAATTGGGGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGC TTACAACGCAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGG ATCGCACGCGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGA AGGACAAAGATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAA CGCGCGGCACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTT ACTCATGGATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAAT TAGCTTTAGATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTG GTGACCCGATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCG CGTTACCGGGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGACAT GCCGTCAGAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGGACACCGGCATG GGTTGAGGGAGCAGTACAAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGG AG

53_AUX2 -- 16..895 of sequence

GGACAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAAC CCACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGG GGACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATG ATTACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTG CGCGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAA GGCGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGG GGGGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGT ACCCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAA GCATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACG AGCATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGA GGTTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGG ACGGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGC CCTACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTG TGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGA AAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG

53_AUX3 -- 15..793 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA

53_premix -- 17..119 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTG

53_premix -- 15..843 of sequence

ATATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATATAT GTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTTGG GTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCAGG CTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCCTC CAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCGGA ACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAACT GGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTGAT TGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTCCA AGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATTCA ACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGGAACTGTGGACCAAACTCGTGC CTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGC

Sequence results for possible auxin producing colony

55_AUX1 -- 18..841 of sequence

ATTTTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAG CATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAA CGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGC CCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGG GGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACG CAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACG CGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAA GATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGC ACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGG ATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTA GATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCG ATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCG GGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCA GAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA

55_AUX2 -- 16..893 of sequence

CAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCCA CGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGGA CTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGATT ACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCGC GAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGGC GACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGGG GTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTACC CGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGCA TTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAGC ATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGGT TCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGACG GACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCCT ACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG

55_AUX3 -- 13..851 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGC

55_premix -- 16..869 of sequence

ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAAA CGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGACC GACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGGC CTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCGT CGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCAG CAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCAG ATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGAT GCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACAG CGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAAC AAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTTC GCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGATT AAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAGC TGTGCGCGTCAGAG

55_premix -- 14..888 of sequence

CTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATA TATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACT TGGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGC AGGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGC CTCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGC GGAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAA ACTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGT GATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTT CCAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATAT TCAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGT GCCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAA ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT

Sequence results for our Auxin Xpress module.

56_AUX1 -- 21..841 of sequence

TTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAGCAT CTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAACGC TGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGCCCA GGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGGGGC GATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACGCAG ATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACGCGT TACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAAGAT CCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGCACA TACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGGATG AGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTAGAT GCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCGATT ACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCGGGC CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA

56_AUX2 -- 16..912 of sequence

GCAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCC ACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGG ACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGAT TACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCG CGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGG CGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGG GGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTAC CCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGC ATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAG CATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGG TTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGAC GGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCC TACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGCCGGTGAG

56_AUX3 -- 15..922 of sequence

GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCACGC TTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA CTCAACGC

56_premix -- 14..930 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGA CAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTG TACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATAT ATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTT GGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCA GGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCC TCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCG GAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAA CTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTG ATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTC CAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATT CAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGTG CCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAAA TTAAGCTTTTACCATCGTGAGGTTTTCGGACAAGTTGGTTTTGGTACAGGGGGTGGGATT CTGATTTTCCAATAGTA

56_premix -- 15..893 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAA ACGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGAC CGACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGG CCTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCG TCGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCA GCAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCA GATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGA TGCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACA GCGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAA CAAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTT CGCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGAT TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC

Sequence results to determine SpeI site problem in sfGFP

17R_premix -- 35..933 of sequence

AGCGAGTCGGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAA AAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGGT AGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGCTCTAGTATCATTACTTATACAGCT CGTCCATACCGTGGGTGATGCCCGCAGCGGTCACAAATTCCAACAGGACCATGTGATCAC GTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTGGGTGCTCAGATAGTGATTATCCGGCA GCAAAACCGGACCGTCACCAATTGGGGTGTTCTGCTGGTAATGGTCGGCCAGTTGGACGC TACCGTCCTCAACATTGTGGCGAATCTTGAAATTGGCCTTGATGCCGTTCTTTTGCTTGT CTGCCGTAATGTAAACATTATGGCTGTTGAAGTTATACTCCAGTTTATGGCCCAGAATGT TACCATCCTCTTTAAAGTCGATACCCTTCAATTCGATACGATTGACCAGCGTGTCACCTT CAAACTTAACCTCTGCGCGAGTTTTGTAGTAGCCATCATCCTTGAAGGAAATGGTACGCT CCTGGACGTAACCCTCCGGCATCGCAGATTTGAAGAAATCGTGTTGTTTCATGTGGTCCG GATAACGCGCAAAACACTGCACGCCATACGTCAACGTCGTCACCAAGGTCGGCCAAGGCA CCGGCAGTTTACCGGTGGTGCAGATGAACTTCAGGGTCAGTTTGCCGTTGGTCGCGTCGC CCTCACCTTCACCGCGAACGCTAAACTTATGACCGTTCACATCGCCGTCCAGCTCGACCA GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA

Sequence results for possible PA2652 producing colony

57.20F_premix -- 14..986 of sequence

CTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAG

57.20R_premix -- 13..1023 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG

Sequence results for possible PA2652 producing colony

57.3F_premix -- 15..985 of sequence

TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGT ACAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGAC CCTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACT GTCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCG CGAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGG CAGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGC CATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTC TCAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGA TCGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGG TTCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAA ACTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTT GGACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCAC CCTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGG GTTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATG ATATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAAC TCGGTGAACTG

57.3R_premix -- 15..1019 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT

Sequence results for possible PA2652 producing colony

57.7F_premix -- 14..987 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAGC

57.7R_premix -- 15..1032 of sequence

GACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTT CGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCG GCCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCA GCTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAA CCGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTT CTGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCG CATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGC TGCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCT GGATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGA CGGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCA GCAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGA CATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCAT GAATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTT GCTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCA TCTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTT CAGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCC CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC

Sequence results for our PA2652 producing construct. Correct.

57.8F_premix -- 15..979 of sequence

TATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACCC TGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTGT CAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGCG AGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGCA GTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCCA TTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCTC AGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGATC GTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGTT CCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAAC TGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTGG ACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACCC TGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGGT TAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGACTCG GTGAC

57.8R_premix -- 14..1022 of sequence

ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG

Dendra2 forward sequencing results after ligation with Ptet and RBS

Dendra_forward_premix -- 15..179 of sequence

TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60a_frwrd_premix -- 15..921 of sequence

ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG CTGCAGG

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60b_frwrd_premix -- 18..928 of sequence

TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT GCAGGCTTCCT

Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange

21+60c_frwrd_premix -- 18..926 of sequence

AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC AGGCTTCCT

Results of corrected SpeI site of sfGFP

sfGFP_reverse_premix -- 20..288 of sequence

ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG GGTGCTCAGATAGTGATTATGCCGGAGCC

Sequence of Anti-holin colony 2

70.2 F_premix -- 25..943 of sequence

AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAGCAACA GCACTTTTTGCCATGCTAGGATTGTCATTTGTTTTATCTCCATCGATTGAAGCGAATGTC GATCCTCATTTTGATAAATTTATGGAATCTGGTATTAGGCACGTTTATATGCTTTTTGAA AATAAAAGCGTAGAATCGTCTGAACAATTCTATAGTTTTATGAGAACGACCTATAAAAAT GACCCGTGCTCTTCTGATTTTGAATGTATAGAGCGAGGCGCGGAGATGGCACAATCATAC GCTAGAATTATGAACATTAAATTGGAGACTGAATAATAACCAGGCATCAAATAAAACGAA AGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT ACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG CCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA AAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA TCGACGCTCAAGTCAGAGT

70.2 R_premix -- 19..1016 of sequence

GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGA GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTC GTTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTA TGATTGTGCCATCTCCGCGCCTCGCTCTATACATTCAAAATCAGAAGAGCACGGGTCATT TTTATAGGTCGTTCTCATAAAACTATAGAATTGTTCAGACGATTCTACGCTTTTATTTTC AAAAAGCATATAAACGTGCCTAATACCAGATTCCATAAATTTATCAAAATGAGGATCGAC ATTCGCTTCAATCGATGGAGATAAAACAAATGACAATCCTAGCATGGCAAAAAGTGCTGT TGCTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGACCGCAGGCTGGCTAGCACTG TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTAT AGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGG CACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTT GAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATAT ACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTT GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT

Sequence of Anti-holin colony 2

70.9 F_premix -- 18..1008 of sequence

ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAACAA CAGCACTTTGGCACAATCATACGCTAGAATTATGAACATTAAATTGGAGACTGAATAATA ACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGT TGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTC TGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCAC CCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGC

70.9 R_premix -- 21..998 of sequence

ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGC TACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAG AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCG TTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTAT GATTGTGCCAAAGTGCTGTTGTTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGAC CGCAGGCTGGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGC GAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTC GTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGT GGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATC ACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGA GAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTT TGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT TCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCT GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTT TTCATCGCTCTGGAGTGA

Sequence of Dendra2 construct. Verified.

71.2F_premix -- 14..961 of sequence ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT

71.2R_premix -- 16..995 of sequence GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT CGGGCACGTAAGAGGTTCCA

Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.

72F_premix -- 15..135 of sequence TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC C

72R_premix -- 20..851 of sequence ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT

J23100-sfGFP composite. Sequence verified.

73F_premix -- 22..1003 of sequence AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT ACTTCGCGTTATGCAGGCTTCC

73R_premix -- 19..338 of sequence GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT GCTGGTAATGGTCGGGTTAC

Retrieved from "http://2011.igem.org/Team:Imperial_College_London/Sequence"