Team:EPF-Lausanne/Our Project/Attributions
From 2011.igem.org
(→pSSB3K1 Pconst-TetR Ptet-LacI) |
|||
(16 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | {{:Team:EPF-Lausanne/Templates/Header|title=Attributions & | + | {{:Team:EPF-Lausanne/Templates/Header|title=Attributions & Contributions}} |
- | + | iGEM being a competition between students, we worked as independently as possible. Once having been taught all the useful procedures, we did them on our own and analyzed the data before presenting them to the instructors and teaching assistants on our weekly meetings. We also planned the experiments needed to be done each week. | |
- | Being a small group, each student specialized on one part of the project, | + | Being a small group, each student specialized on one part of the project, such as using the MITOMI or creating the T7 promoter variants. This allowed us to spend less time on learning the procedures; especially true for the microfluidics setups. The different parts of the project are detailed below, with the name of the student that worked on each task. The teaching assistants were assigned to the different tasks based on their personal competence; this does not mean that they completed the task, but rather that they taught us how to do it and answered our questions relative to it. |
== TetR mutants == | == TetR mutants == | ||
Line 14: | Line 14: | ||
|- | |- | ||
| Primer design | | Primer design | ||
- | | | + | | Clara |
| Alina | | Alina | ||
|- | |- | ||
Line 29: | Line 29: | ||
| | | | ||
|} | |} | ||
- | |||
=== MITOMI === | === MITOMI === | ||
Line 42: | Line 41: | ||
|} | |} | ||
- | |||
- | |||
== T7 promoter variants == | == T7 promoter variants == | ||
Line 67: | Line 64: | ||
| | | | ||
|} | |} | ||
+ | |||
+ | == T7 Lysis == | ||
+ | {| | ||
+ | ! Which task | ||
+ | ! Student(s) | ||
+ | ! Assistant(s) | ||
+ | |- | ||
+ | | Primer design | ||
+ | | Alessandro | ||
+ | | Henrike | ||
+ | |- | ||
+ | | PCR and Assembly | ||
+ | | Alessandro, Vincent | ||
+ | | Henrike | ||
+ | |- | ||
+ | | Characterization | ||
+ | | Vincent | ||
+ | | Henrike | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | |||
Line 82: | Line 102: | ||
| Cloning | | Cloning | ||
| Vincent | | Vincent | ||
- | | | + | | Irina |
|- | |- | ||
| In vivo characterization | | In vivo characterization | ||
| Nadine, Alessandro | | Nadine, Alessandro | ||
| | | | ||
- | |||
- | |||
- | |||
- | |||
|} | |} | ||
- | |||
=== J61002 Plac-RFP === | === J61002 Plac-RFP === | ||
Line 111: | Line 126: | ||
| Nadine | | Nadine | ||
| | | | ||
+ | |- | ||
+ | | Biobrick of Plac | ||
+ | | Nadine | ||
+ | | Sequence from Henrike | ||
|} | |} | ||
Line 140: | Line 159: | ||
| Primer design | | Primer design | ||
| Alessandro | | Alessandro | ||
- | | | + | | Henrike |
|- | |- | ||
| Cloning | | Cloning | ||
| Nadine, Alessandro | | Nadine, Alessandro | ||
- | | | + | | Irina, Henrike |
|- | |- | ||
| In vivo characterization | | In vivo characterization | ||
| Nadine | | Nadine | ||
- | | | + | | Henrike |
|} | |} | ||
Line 178: | Line 197: | ||
|- | |- | ||
| Chip design | | Chip design | ||
+ | | Clara, Douglas | ||
+ | | Sebastian | ||
+ | |- | ||
+ | | Wafer fabrication | ||
| Clara | | Clara | ||
| | | | ||
Line 185: | Line 208: | ||
| Henrike | | Henrike | ||
|- | |- | ||
- | | | + | | Building the game setup |
| Douglas | | Douglas | ||
| | | | ||
|- | |- | ||
- | | | + | | Programming the web interface |
| Douglas | | Douglas | ||
| | | | ||
Line 202: | Line 225: | ||
| Chip design | | Chip design | ||
| | | | ||
- | | Existing design | + | | Existing design |
|- | |- | ||
- | | Chip | + | | Chip fabrication |
- | | Lilia | + | | Lilia, Clara |
| | | | ||
|- | |- | ||
Line 220: | Line 243: | ||
! Assistant(s) | ! Assistant(s) | ||
|- | |- | ||
- | | | + | | Layout |
| Douglas | | Douglas | ||
| | | | ||
|- | |- | ||
- | | Logo | + | | Logo design |
| Solange | | Solange | ||
| | | | ||
|- | |- | ||
- | | Team banner | + | | Team banner, logo colouring |
| Douglas | | Douglas | ||
| | | | ||
|- | |- | ||
- | | Specialized | + | | Safety Page |
+ | |||
+ | | Vincent | ||
+ | | | ||
+ | |- | ||
+ | | Specialized pages | ||
| According to our work | | According to our work | ||
| | | | ||
|- | |- | ||
- | | General | + | | General pages |
| Various team members | | Various team members | ||
| | | | ||
+ | |- | ||
+ | | Graphing and Reporting | ||
+ | | Douglas, Vincent | ||
+ | | Irina | ||
+ | |- | ||
+ | | Attributions page | ||
+ | | Nadine | ||
+ | | | ||
+ | |} | ||
+ | |||
+ | == Misc == | ||
+ | |||
+ | |||
+ | {| | ||
+ | ! Which task | ||
+ | ! Student(s) | ||
+ | ! Assistant(s) | ||
+ | |- | ||
+ | | Agar plates | ||
+ | | Lilia | ||
+ | | | ||
+ | |- | ||
+ | | Antibiotics aliquots | ||
+ | | Lilia | ||
+ | | | ||
+ | |- | ||
+ | | Lab entertainment | ||
+ | | Minnie mouse, the Hawaiian girl, the Pirate | ||
+ | | | ||
+ | |- | ||
+ | | Stress level managers | ||
+ | | | ||
+ | | Bart, Sebastian | ||
+ | |- | ||
+ | | Cakes | ||
+ | | | ||
+ | | Matt, the LBNC lab | ||
|} | |} | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Latest revision as of 17:20, 21 September 2011
Attributions & Contributions
iGEM being a competition between students, we worked as independently as possible. Once having been taught all the useful procedures, we did them on our own and analyzed the data before presenting them to the instructors and teaching assistants on our weekly meetings. We also planned the experiments needed to be done each week.
Being a small group, each student specialized on one part of the project, such as using the MITOMI or creating the T7 promoter variants. This allowed us to spend less time on learning the procedures; especially true for the microfluidics setups. The different parts of the project are detailed below, with the name of the student that worked on each task. The teaching assistants were assigned to the different tasks based on their personal competence; this does not mean that they completed the task, but rather that they taught us how to do it and answered our questions relative to it.
Contents |
TetR mutants
Site-directed mutagenesis
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Clara | Alina |
Cloning | Douglas, Vincent | Alina |
In vivo characterization | Nadine | |
Biobrick | Lilia |
MITOMI
Which task | Student(s) | Assistant(s) |
---|---|---|
In vitro characterization | Lilia | Alina, Matt |
T7 promoter variants
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Alessandro | Henrike |
Cloning | Alessandro, Vincent | |
In vivo characterization | Alessandro, Vincent | |
Biobrick | Nadine |
T7 Lysis
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Alessandro | Henrike |
PCR and Assembly | Alessandro, Vincent | Henrike |
Characterization | Vincent | Henrike |
Reporter plasmids
J61002 Ptet-RFP
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Henrike | |
Cloning | Vincent | Irina |
In vivo characterization | Nadine, Alessandro |
J61002 Plac-RFP
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Nadine | |
Cloning | Nadine, Alessandro | |
In vivo characterization | Nadine | |
Biobrick of Plac | Nadine | Sequence from Henrike |
J61002 Plac-lysis
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Nadine | |
Cloning | Nadine, Alessandro | |
In vivo characterization |
pSB3K1 Pconst-TetR
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Alessandro | Henrike |
Cloning | Nadine, Alessandro | Irina, Henrike |
In vivo characterization | Nadine | Henrike |
pSB3K1 Pconst-TetR Ptet-LacI
Which task | Student(s) | Assistant(s) |
---|---|---|
Primer design | Nadine | |
Cloning | Nadine | |
In vivo characterization | Nadine |
Microfluidics chips
Worm chip
Which task | Student(s) | Assistant(s) |
---|---|---|
Chip design | Clara, Douglas | Sebastian |
Wafer fabrication | Clara | |
Chip fabrication | Clara | Henrike |
Building the game setup | Douglas | |
Programming the web interface | Douglas |
MITOMI chip
Which task | Student(s) | Assistant(s) |
---|---|---|
Chip design | Existing design | |
Chip fabrication | Lilia, Clara | |
Experiments | Lilia | Alina, Matt |
Wiki
Which task | Student(s) | Assistant(s) |
---|---|---|
Layout | Douglas | |
Logo design | Solange | |
Team banner, logo colouring | Douglas | |
Safety Page | Vincent | |
Specialized pages | According to our work | |
General pages | Various team members | |
Graphing and Reporting | Douglas, Vincent | Irina |
Attributions page | Nadine |
Misc
Which task | Student(s) | Assistant(s) |
---|---|---|
Agar plates | Lilia | |
Antibiotics aliquots | Lilia | |
Lab entertainment | Minnie mouse, the Hawaiian girl, the Pirate | |
Stress level managers | Bart, Sebastian | |
Cakes | Matt, the LBNC lab |