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West Point/11 August 2011
From 2011.igem.org
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<div id="frame"><h1 style="font-size:70px;">09AUGUST11</h1><p style="font-size:20px;">Develop procedure for making buffer solutions for AP and X-phos; collect materials <br /> | <div id="frame"><h1 style="font-size:70px;">09AUGUST11</h1><p style="font-size:20px;">Develop procedure for making buffer solutions for AP and X-phos; collect materials <br /> | ||
| - | o BCIP Buffer <br /> | + |  o BCIP Buffer <br />   |
| - | 0.1 M Tris <br /> | + | 0.1 M Tris <br />   |
| + | 0.1M NaCl <br />   | ||
| + | 5 mM MgCl2 <br />   | ||
| + | Add 0.1 M HCl until pH reaches 9.5 <br /><br />  | ||
| - | + | o A.P. Buffer <br />    | |
| - | + | 10 mM Tris<br />   | |
| - | + | 5 mM MgCl2<br />   | |
| - | + | 0.2 mM ZnCl2<br />   | |
| - | + | ||
| - | + | ||
| - | o A.P. Buffer <br /> | + | |
| - | + | ||
| - | + | ||
| - | 10 mM Tris<br /> | + | |
| - | 5 mM MgCl2<br /> | + | |
| - | 0.2 mM ZnCl2<br /> | + | |
Add 0.1 M HCl until pH reaches 8.0 | Add 0.1 M HCl until pH reaches 8.0 | ||
</p> | </p> | ||
</div> | </div> | ||
Latest revision as of 00:55, 15 September 2011
Click on a date to see a summary of labwork done on that day (red pages are the only ones that have an entry). Up-to-date to 5September11. Best viewed with Mozilla Firefox. Props to the 2008 Edinburgh team, we used quite a bit of their code for this.
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09AUGUST11
Develop procedure for making buffer solutions for AP and X-phos; collect materials
o BCIP Buffer
0.1 M Tris
0.1M NaCl
5 mM MgCl2
Add 0.1 M HCl until pH reaches 9.5
o A.P. Buffer
10 mM Tris
5 mM MgCl2
0.2 mM ZnCl2
Add 0.1 M HCl until pH reaches 8.0
